van de Giessen A W, van Wijk E M
Clinical Chemistry Department, Military Hospital Dr. A. Mathijsen, Utrecht, The Netherlands.
J Clin Chem Clin Biochem. 1990 Sep;28(9):605-9. doi: 10.1515/cclm.1990.28.9.605.
An improved assay for quantification of urinary porphyrins by use of second-derivative spectroscopy is described. A new method for calculation of the porphyrin concentration is developed and the whole procedure is computerized. Acidified urine samples can be assayed within a few minutes by using this method. Precision and recoveries for both uro- and coproporphyrin are good. The method is presented as a very fast and accurate assay for the screening and quantification of urinary porphyrins.
描述了一种利用二阶导数光谱法改进的尿卟啉定量分析方法。开发了一种计算卟啉浓度的新方法，并将整个过程计算机化。使用该方法，酸化的尿液样本可在几分钟内进行分析。尿卟啉和粪卟啉的精密度和回收率都很好。该方法是一种用于尿卟啉筛查和定量的非常快速且准确的分析方法。
