Christensen N G, Johannessen B, Romslo I
Clin Chim Acta. 1977 Dec 15;81(3):229-35. doi: 10.1016/0009-8981(77)90053-5.
A procedure has been developed for the separation and quantitative assay of urinary porphyrins. Urine was directly diluted to a final concentration of 1 MHCl and the amount of porphyrins was determined from the peak-to-trough height deflection of the first derivatives of the absorption spectrum in the region of the Soret band. The ratio uro-/coproporphyrin was determined from the wavelength at which the spectrum intercepted the baseline. The specificity (as shown by a correlation coefficient of 0.99 compared to the method of Doss and Schmidt (1971) Z. Klin. Chem. Klin. Biochem. 9, 415-418), precision (coefficient of variation 5.2 percent) and sensitivity (lower detection limit approx. 0.01 mumol/l) of the present method were highly sufficient to estimate total and different porphyrins in the routine laboratory.
已开发出一种用于分离和定量测定尿卟啉的方法。将尿液直接稀释至最终浓度为1 M HCl，并根据Soret带区域吸收光谱一阶导数的峰谷高度偏移来测定卟啉的含量。尿卟啉/粪卟啉的比值由光谱与基线相交处的波长确定。本方法的特异性（与多斯和施密特（1971年）的方法相比，相关系数为0.99，《临床化学与临床生物化学杂志》9, 415 - 418）、精密度（变异系数5.2%）和灵敏度（检测下限约为0.01 μmol/l）足以在常规实验室中估算总卟啉和不同卟啉。
