Prommaban Adchara, Kodchakorn Kanchanok, Kongtawelert Prachya, Banjerdpongchai Ratana
Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Asian Pac J Cancer Prev. 2012;13(5):1977-81. doi: 10.7314/apjcp.2012.13.5.1977.
Houttuynia cordata Thunb (HCT) is a native herb found in Southeast Asia which features various pharmacological activities against allergy, inflammation, viral and bacterial infection, and cancer. The aims of this study were to determine the cytotoxic effect of 6 fractions obtained from silica gel column chromatography of alcoholic HCT extract on human leukemic Molt-4 cells and demonstrate mechanisms of cell death. Six HCT fractions were cytotoxic to human lymphoblastic leukemic Molt-4 cells in a dose-dependent manner by MTT assay, fraction 4 exerting the greatest effects. Treatment with IC50 of HCT fraction 4 significantly induced Molt-4 apoptosis detected by annexinV-FITC/propidium iodide for externalization of phosphatidylserine to the outer layer of cell membrane. The mitochondrial transmembrane potential was reduced in HCT fraction 4-treated Molt-4 cells. Moreover, decreased expression of Bcl-xl and increased levels of Smac/Diablo, Bax and GRP78 proteins were noted on immunoblotting. In conclusion, HCT fraction 4 induces Molt-4 apoptosis cell through an endoplasmic reticulum stress pathway.
鱼腥草是一种原产于东南亚的草本植物，具有多种药理活性，可对抗过敏、炎症、病毒和细菌感染以及癌症。本研究的目的是确定从鱼腥草乙醇提取物的硅胶柱色谱中获得的6个馏分对人白血病Molt-4细胞的细胞毒性作用，并阐明细胞死亡机制。通过MTT法检测，6个鱼腥草馏分对人淋巴细胞白血病Molt-4细胞具有剂量依赖性的细胞毒性作用，其中馏分4的作用最为显著。用鱼腥草馏分4的IC50处理可显著诱导Molt-4细胞凋亡，通过膜联蛋白V-FITC/碘化丙啶检测细胞膜外层磷脂酰丝氨酸的外化来确定。在经鱼腥草馏分4处理的Molt-4细胞中，线粒体跨膜电位降低。此外，免疫印迹显示Bcl-xl表达降低，Smac/Diablo、Bax和GRP78蛋白水平升高。总之，鱼腥草馏分4通过内质网应激途径诱导Molt-4细胞凋亡。
