China Medical University, No 91, Hsueh-Shih Road, Taichung 404, Taiwan, ROC.
Anticancer Res. 2010 Sep;30(9):3549-56.
It is reported that Houttuynia cordata Thunb. (HCT), a traditional Chinese herbal medicine, has many biological properties such as antiviral, antibacterial and antileukemic activities. However, the molecular mechanisms of cytotoxicity and apoptosis in human primary colorectal cancer cells are not clear. In this study, whether HCT induced cytotoxicity in primary colorectal cancer cells obtained from three patients was investigated. The results indicated that HCT inhibited growth of cancer cells in a dose-dependent manner. After treatment with HCT (250 μg/ml) for 24 h, cells exhibited chromatin condensation (an apoptotic characteristic). HCT increased reactive oxygen species (ROS) production and decreased the mitochondrial membrane potential (ΔΨ(m)) in examined cells. Mitochondria-dependent apoptotic signaling pathway was shown to be involved as determined by increase in the levels of cytochrome c, Apaf-1, and caspase-3 and -9. The decrease in the level of ΔΨ(m) was associated with an increase in the BAX/BCL-2 ratio which led to activation of caspase-9 and -3. Based on our results, HCT induced apoptotic cell death in human primary colorectal cancer cells through a mitochondria-dependent signaling pathway.
据报道，鱼腥草（Houttuynia cordata Thunb.，HCT）作为一种传统中药，具有多种生物特性，如抗病毒、抗菌和抗白血病活性。然而，人原发性结直肠癌细胞中细胞毒性和细胞凋亡的分子机制尚不清楚。在本研究中，我们研究了鱼腥草是否会诱导来自 3 名患者的原发性结直肠癌细胞的细胞毒性。结果表明，鱼腥草以剂量依赖的方式抑制癌细胞生长。用鱼腥草（250μg/ml）处理 24 小时后，细胞出现染色质浓缩（凋亡特征）。鱼腥草增加了活性氧（ROS）的产生，并降低了被检细胞中的线粒体膜电位（ΔΨ（m））。通过增加细胞色素 c、Apaf-1 和 caspase-3 和 -9 的水平，表明涉及线粒体依赖性凋亡信号通路。ΔΨ（m）的降低与 BAX/BCL-2 比值的增加有关，这导致 caspase-9 和 -3 的激活。基于我们的结果，鱼腥草通过线粒体依赖性信号通路诱导人原发性结直肠癌细胞的凋亡性细胞死亡。
