Yang Li, Sun Ming-Jun, Liu Jing-Wei, Xu Qian, Yuan Yuan
Department of Gastroenterology, the First Affiliated Hospital of China Medical University, Shenyang, China E-mail :
Asian Pac J Cancer Prev. 2013;14(12):7467-72. doi: 10.7314/apjcp.2013.14.12.7467.
Polymorphisms of genes encoding cytokines could be potential biomarkers to predict risk of gastric cancer (GC). Here, we investigated the association between the IL-6 -6331 (T/C, rs10499563) polymorphism in its promoter region and GC risk.
In this case-control study of 215 GC cases and 518 non-cancer controls, the IL-6 -6331 (T/C, rs10499563) polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Individuals with the TC or CC genotype were associated with a significantly decreased risk of GC (OR=0.710, 95%CI: 0.504-0.999, P=0.049) compared with TT wild-type carriers. Ther C allele was also associated with significantly decreased risk of GC (OR=0.715, 95%CI: 0.536- 0.954, P=0.023) compared with the T allele. In the stratification analysis, TC or CC genotypes were associated with significantly decreased GC risk in subgroups of males, people older than 60, and H. pylori-positive cases. However, no significant interaction was observed for TC or CC genotypes with H. pylori infection. On stratification with the Lauren classification, TC or CC genotypes were associated with significantly decreased risk of diffuse- type GC (OR=0.497, 95%CI: 0.266-0.925, P=0.027), also in subgroups of males, people older than 60, and H. pylori-positive cases.
The IL-6 -6331 (T/C, rs10499563) polymorphism is associated with genetic susceptibility of GC and may have the potential to predict GC risk.
编码细胞因子的基因多态性可能是预测胃癌（GC）风险的潜在生物标志物。在此，我们研究了白细胞介素-6（IL-6）启动子区域-6331（T/C，rs10499563）多态性与GC风险之间的关联。
在这项包含215例GC病例和518例非癌症对照的病例对照研究中，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对IL-6 -6331（T/C，rs10499563）多态性进行基因分型。
与TT野生型携带者相比，TC或CC基因型个体的GC风险显著降低（OR = 0.710，95%CI：0.504 - 0.999，P = 0.049）。与T等位基因相比，C等位基因也与GC风险显著降低相关（OR = 0.715，95%CI：0.536 - 0.954，P = 0.023）。在分层分析中，TC或CC基因型在男性、60岁以上人群和幽门螺杆菌阳性病例亚组中与GC风险显著降低相关。然而，未观察到TC或CC基因型与幽门螺杆菌感染之间存在显著相互作用。根据劳伦分类法进行分层时，TC或CC基因型在男性、60岁以上人群和幽门螺杆菌阳性病例亚组中也与弥漫型GC风险显著降低相关（OR = 0.497，95%CI：0.266 - 0.925，P = 0.027）。
IL-6 -6331（T/C，rs10499563）多态性与GC的遗传易感性相关，可能具有预测GC风险的潜力。
