Qu Yue, Sun Honglei, Sun Meiyu, Li Delong, Qin Liting, Gao Yulong, Wang Xiaomei, Liu Sidang
Shandong Agricultural University College of Animal Science and Veterinary Medicine Taian 271018 P. R. China Chinese Academy of Agricultural Sciences Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute Harbin, Heilongjiang P. R. China Liaoning Medical University College of Animal Husbandry and Veterinary Science Jinzhou City, Liaoning P. R. China.
Acta Vet Hung. 2012 Sep;60(3):343-54. doi: 10.1556/AVet.2012.029.
Avian leukosis virus subgroup J (ALV-J) can cause a variety of neoplasms, including mainly myeloid leukosis (myelocytomatosis) and nephromas. Other tumours, such as histiocytic sarcoma (HS), haemangiosarcoma and mesothelioma, may also develop. In a previous article we described a case in which myeloid leukosis, haemangiomas and leiomyosarcomas appeared simultaneously in a commercial layer flock with infection by ALV-J. The present research was completed to understand the molecular characteristics of the ALV-J strain that induced clinical myeloid leukosis, haemangiomas and leiomyosarcomas. Two strains of ALV-J (SDAU1001 and SDAU1002) were isolated and identified, and their full-length sequences were analysed. The complete genome nucleotide sequences of these two isolates were different in length, 7652 nt and 7636 nt, respectively. They shared 98.9% identity with each other, and 93.4% to 97.8% nucleotide identity to the reference ALV-J isolates. A 19-nucleotide repeat sequence was identified in the primer binding site (PBS) leader region of isolate SDAU1001. A base substitution mutation (base 15 C-T) in this insertion was identified. However, the identical insertion at the same site was not found in SDAU1002. The gag and pol genes of the two viruses were more conserved than the env gene. One key deletion in the E element was a common feature of SDAU1001 and SDAU1002. SDAU1001 and SDAU1002, possibly recombinants of ALV-J and another avian retrovirus, may share the same ancestor. Co-infection by SDAU1001 and SDAU1002 isolates is a possible explanation why myeloid leukosis, haemangiomas, and leiomyosarcomas appeared simultaneously in the same commercial layer flock.
J亚群禽白血病病毒（ALV-J）可引发多种肿瘤，主要包括骨髓性白血病（骨髓细胞瘤病）和肾瘤。其他肿瘤，如组织细胞肉瘤（HS）、血管肉瘤和间皮瘤，也可能发生。在之前的一篇文章中，我们描述了一个案例，在一个感染了ALV-J的商品蛋鸡群中，同时出现了骨髓性白血病、血管瘤和平滑肌肉瘤。本研究旨在了解引发临床骨髓性白血病、血管瘤和平滑肌肉瘤的ALV-J毒株的分子特征。分离并鉴定了两株ALV-J（SDAU1001和SDAU1002），并对其全长序列进行了分析。这两个分离株的完整基因组核苷酸序列长度不同，分别为7652 nt和7636 nt。它们彼此之间的同源性为98.9%，与参考ALV-J分离株的核苷酸同源性为93.4%至97.8%。在分离株SDAU1001的引物结合位点（PBS）前导区域鉴定出一个19个核苷酸的重复序列。在该插入序列中鉴定出一个碱基替换突变（第15位碱基C-T）。然而，在SDAU1002中未在同一位置发现相同的插入序列。这两种病毒的gag和pol基因比env基因更保守。E元件中的一个关键缺失是SDAU1001和SDAU1002的共同特征。SDAU1001和SDAU1002可能是ALV-J与另一种禽逆转录病毒的重组体，可能有共同的祖先。SDAU1001和SDAU1002分离株的共同感染可能是同一商品蛋鸡群中同时出现骨髓性白血病、血管瘤和平滑肌肉瘤的一个原因。
