College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, China.
College of Environmental and Resource Sciences, Henan Agricultural University, Zhengzhou, 450000, China.
BMC Vet Res. 2020 Jun 29;16(1):218. doi: 10.1186/s12917-020-02430-3.
Studies have shown that some viral infections cause structural changes in the intestinal microflora, but little is known about the effects of tumorigenic viral infection on the intestinal microflora of chickens.
A 29-week commercial layer flock positive for avian leukosis virus-J (ALV-J), Marek's disease virus (MDV) and avian reticuloendotheliosis virus (REV) was selected, and fresh fecal samples were collected and examined for the composition of the gut microflora by Illumina sequencing of the V3-V4 region of the 16S rRNA gene. The operational taxonomic units (OTUs) of the fecal microbiota differentiated the chickens infected with only ALV-J and those coinfected with ALV-J and MDV or REV from infection-negative chickens. The enrichment and diversity of cloacal microflora in chickens infected with ALV-J alone were slightly different from those in the infection-negative chickens. However, the diversity of cloacal microflora was significantly increased in chickens coinfected with both ALV-J and MDV or REV.
The intestinal microbiota was more strongly disturbed in chickens after coinfection with ALV-J and MDV or REV than after infection with ALV-J alone, and there may be underlying mechanisms by which the capacity for the stabilization of the intestinal flora was impaired due to viral infection and tumorigenesis.
研究表明，某些病毒感染会导致肠道微生物群落的结构发生变化，但对于致瘤性病毒感染对鸡肠道微生物群落的影响知之甚少。
选择了一个 29 周龄的商业蛋鸡群，该鸡群对禽白血病病毒-J（ALV-J）、马立克氏病病毒（MDV）和禽网状内皮组织增生病病毒（REV）呈阳性，采集新鲜粪便样本，并通过 Illumina 对 16S rRNA 基因的 V3-V4 区进行测序，检测肠道微生物群落的组成。粪便微生物群的操作分类单元（OTUs）将仅感染 ALV-J 的鸡与同时感染 ALV-J 和 MDV 或 REV 的鸡以及未感染的鸡区分开来。单独感染 ALV-J 的鸡的泄殖腔微生物群的丰度和多样性与未感染的鸡略有不同。然而，同时感染 ALV-J 和 MDV 或 REV 的鸡的泄殖腔微生物群的多样性显著增加。
与单独感染 ALV-J 相比，ALV-J 和 MDV 或 REV 共同感染后鸡的肠道微生物群受到更强烈的干扰，由于病毒感染和肿瘤发生，肠道菌群稳定能力受损可能存在潜在机制。
