Wojtkiewicz Joanna, Gonkowski Sławomir, Bladowski Marek, Majewski Mariusz
University of Warmia and Mazury Division of Neurosurgery, Department of Neurology and Neurosurgery ul. Warszawska 30 10-082 Olsztyn Poland.
Acta Vet Hung. 2012 Sep;60(3):371-81. doi: 10.1556/AVet.2012.032.
The aim of this study was to investigate the distribution and the number of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) neurons and the co-localisation of CART with substance P (SP), somatostatin (SOM), nitric oxide synthase (NOS) and vasoactive intestinal polypeptide (VIP) within the enteric nervous system (ENS) in the porcine small intestine. Accordingly, the myenteric plexus (MP), outer submucous plexus (OSP) and inner submucous plexus (ISP) of the small intestine (duodenum, jejunum and ileum) were studied by double-labelling immunofluorescence technique. CART-LI neurons were observed in all gut fragments and all types of intramural plexuses studied and amounted from 0.2 ± 0.1% in the ISP of ileum to 22.4 ± 2.4% in the MP of this segment. The co-localisation of CART and NOS or/and VIP was observed depending on the segment of the gut and the complexity of the intramural plexus. On the other hand, during this study the co-localisation of CART and SOM or/and SP was not observed. The present study, for the first time, presents a detailed description of the CART distribution pattern and co-localisation with other neuromodulators within the ENS of the porcine small intestine.
本研究旨在调查猪小肠肠神经系统（ENS）中可卡因和苯丙胺调节转录物样免疫反应性（CART-LI）神经元的分布和数量，以及CART与P物质（SP）、生长抑素（SOM）、一氧化氮合酶（NOS）和血管活性肠肽（VIP）的共定位情况。为此，采用双标免疫荧光技术对小肠（十二指肠、空肠和回肠）的肌间神经丛（MP）、外粘膜下神经丛（OSP）和内粘膜下神经丛（ISP）进行了研究。在所有研究的肠段和所有类型的壁内神经丛中均观察到CART-LI神经元，其数量在回肠ISP中为0.2±0.1%，在该段MP中为22.4±2.4%。根据肠段和壁内神经丛的复杂性，观察到CART与NOS或/和VIP的共定位情况。另一方面，在本研究中未观察到CART与SOM或/和SP的共定位情况。本研究首次详细描述了猪小肠ENS中CART的分布模式及其与其他神经调质的共定位情况。
