Department of Clinical Physiology Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 13, 10-719 Olsztyn, Poland.
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland.
J Diabetes Res. 2018 Jul 24;2018:4735659. doi: 10.1155/2018/4735659. eCollection 2018.
One of the most frequently reported disorders associated with diabetes is gastrointestinal (GI) disturbance. Although pathogenesis of these complications is multifactorial, the complicity of the enteric nervous system (ENS) in this respect has significant importance. Therefore, this paper analysed changes in substance P- (SP-), calcitonin gene-related peptide- (CGRP-), and leu5-enkephalin- (L-ENK-) like immunoreactivity (LI) in enteric stomach neurons caused by chemically induced diabetes in a porcine model. Using double immunofluorescent labelling, it was found that acute hyperglycaemia led to significant changes in the chemical coding of stomach enteric neurons. Generally, the response to artificially inducted diabetes depended on the "kind" of enteric plexus as well as the stomach region studied. A clear increase in the percentage of neurons immunoreactive to SP and CGRP was visible in the myenteric plexus (MP) in the antrum, corpus, and pylorus as well as in the submucosal plexus (SmP) in the corpus. For L-ENK, an increase in the number of L-ENK-LI neurons was observed in the MP of the antrum and SmP in the corpus, while in the MP of the corpus and pylorus, a decrease in the percentage of L-ENK-LI neurons was noted.
与糖尿病相关的最常见的报告紊乱之一是胃肠道（GI）紊乱。虽然这些并发症的发病机制是多因素的，但肠神经系统（ENS）在这方面的复杂性具有重要意义。因此，本文分析了化学诱导的猪模型糖尿病对胃肠神经元中 P 物质（SP）、降钙素基因相关肽（CGRP）和亮氨酸脑啡肽（L-ENK）样免疫反应性（LI）的变化。使用双重免疫荧光标记，发现急性高血糖导致胃肠神经元的化学编码发生显著变化。一般来说，对人工诱导的糖尿病的反应取决于肠丛的“类型”以及研究的胃区。在胃窦、胃体和胃底的肌间神经丛（MP）以及胃体的黏膜下神经丛（SmP）中，可见对 SP 和 CGRP 免疫反应性神经元的百分比明显增加。对于 L-ENK，在胃窦的 MP 和胃体的 SmP 中观察到 L-ENK-LI 神经元数量增加，而在胃体和胃底的 MP 中，L-ENK-LI 神经元的百分比减少。
