Department of Life Sciences, Chonbuk National University, Jeonju-si, 561-756, Republic of Korea.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1653-1657. doi: 10.1099/ijs.0.045203-0. Epub 2012 Aug 17.
A taxonomic study was carried out on a novel bacterial strain, designated MIC3010(T), which was isolated from a freshwater pond in Jeonju, Republic of Korea. Cells of the isolate were Gram-stain-negative, rod-shaped and non-motile. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the family Flavobacteriaceae, with Flavobacterium haoranii LQY-7(T) as its closest relative, with a similarity of 94.2 %. The predominant fatty acids of strain MIC3010(T) were iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipid profile of strain MIC3010(T) revealed the presence of phosphatidylethanolamine (PE) and one unidentified lipid (L1) as major components. In addition, two aminolipids (AL1, AL2) and one glycolipid were present in small amounts. The DNA G+C content of the strain was 41.0 mol%. The strain contained MK-6 as the major quinone and sym-homospermidine as the predominant polyamine. On the basis of the evidence presented, it is concluded that strain MIC3010(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium fontis sp. nov. is proposed. The type strain is MIC3010(T) ( = KACC 16593(T) = JCM 18212(T)).
对一株新型细菌菌株 MIC3010(T) 进行了分类学研究，该菌株从韩国全州的一个淡水池塘中分离得到。分离株的细胞革兰氏染色阴性，呈杆状，不运动。比较 16S rRNA 基因序列表明，该分离株属于黄杆菌科，与豪氏黄杆菌 LQY-7(T) 的亲缘关系最近，相似度为 94.2%。菌株 MIC3010(T) 的主要脂肪酸为 iso-C15:1 G、iso-C15:0 和 iso-C17:0 3-OH。菌株 MIC3010(T) 的极性脂谱显示存在磷脂酰乙醇胺 (PE) 和一种未鉴定的脂 (L1) 作为主要成分。此外，还存在两种氨基脂 (AL1、AL2) 和一种少量的糖脂。该菌株的 DNA G+C 含量为 41.0 mol%。该菌株含有 MK-6 作为主要醌和 sym-homospermidine 作为主要多胺。基于提出的证据，结论是菌株 MIC3010(T) 代表黄杆菌属的一个新型物种，建议将其命名为 Flavobacterium fontis sp. nov.。其模式菌株为 MIC3010(T) ( = KACC 16593(T) = JCM 18212(T))。
