Joung Yochan, Kang Heeyoung, Kim Haneul, Kim Tae-Su, Han Ji-Hye, Kim Seung Bum, Ahn Tae-Seok, Joh Kiseong
Department of Biological Science, Inha University, Incheon 402-751, Republic of Korea.
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, 449-791, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Jan;66(1):365-370. doi: 10.1099/ijsem.0.000727. Epub 2015 Oct 30.
A Gram-stain-negative, motile by gliding, yellow-pigmented bacterial strain, designated KNUS1T, was isolated from Lake Paro in Korea. The phylogenetic tree based on 16S rRNA gene sequences showed that strain KNUS1T formed a distinct lineage within the genus Flavobacterium. Strain KNUS1T was closely related to Flavobacterium cheonhonense ARSA-15T (96.8 %16S rRNA gene sequence similarity), Flavobacterium pectinovorum DSM 6368T (96.3 %) and Flavobacterium dankookense ARSA-19T (96.1 %). The major fatty acids of strain KNUS1T were iso-C15 : 0 and iso-C15 : 1 G. The major polyamine was sym-homospermidine. The major polar lipids of strain KNUS1T were phosphatidylethanolamine, five unidentified aminolipids and three unidentified polar lipids. The major respiratory'quinone was menaquinone 6 (MK-6). The DNA G+C content of strain KNUS1T was 34.2 mol%. On the basis of the evidence presented in this study, strain KNUS1T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium paronense sp. nov. is proposed. The type strain is KNUS1T ( = KACC 17692T = CECT 8460T).
从韩国巴罗湖分离出一株革兰氏染色阴性、靠滑动运动、产黄色色素的细菌菌株，命名为KNUS1T。基于16S rRNA基因序列构建的系统发育树表明，菌株KNUS1T在黄杆菌属内形成了一个独特的分支。菌株KNUS1T与cheonhonense黄杆菌ARSA - 15T（16S rRNA基因序列相似性为96.8%）、果胶黄杆菌DSM 6368T（96.3%）和丹国黄杆菌ARSA - 19T（96.1%）密切相关。菌株KNUS1T的主要脂肪酸为异C15 : 0和异C15 : I G。主要多胺为对称高亚精胺。菌株KNUS1T的主要极性脂为磷脂酰乙醇胺、5种未鉴定的氨基脂和3种未鉴定的极性脂。主要呼吸醌为甲基萘醌6（MK - 6）。菌株KNUS1T的DNA G+C含量为34.2 mol%。基于本研究提供的证据，菌株KNUS1T代表黄杆菌属的一个新物种，为此提出名为巴罗黄杆菌（Flavobacterium paronense）的新种。模式菌株为KNUS1T（=KACC 17692T = CECT 8460T）。
