Key Lab of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1621-1626. doi: 10.1099/ijs.0.044271-0. Epub 2012 Aug 17.
A Gram-negative, short-rod-shaped and non-motile bacterium, strain 12C11(T), was isolated from an oil-degrading consortium, enriched from the Fugong mangrove sediment, Fujian Province of China. Optimum growth was observed at 25 °C, at pH 7.5 and with 4 % (w/v) NaCl. Comparative 16S rRNA gene sequence analysis demonstrated that it shared highest similarity with members of the genus Algoriphagus (97.5-93.4 %), exhibiting 97.5 % sequence similarity to Algoriphagus ornithinivorans IMSNU 14014(T), followed by Algoriphagus vanfongensis KMM 6241(T) (97.2 %); it shared <96.0 % sequence similarity with other members of the genus. Levels of nucleotide sequence similarity between gyrB (DNA gyrase subunit B) genes of strain 12C11(T) and A. vanfongensis KMM 6241(T), A. ornithinivorans IMSNU 14014(T), Algoriphagus marincola SW-2(T) and Algoriphagus hitonicola 7-UAH(T) were 78.8, 78.6, 75.6 and 77.4 %, respectively. Phylogenetic trees based on these housekeeping genes showed that strain 12C11(T) and other Algoriphagus strains formed a distinct lineage. The dominant fatty acids were iso-C15 : 0 (32.1 %), C16 : 1ω7c/C16 : 1ω6c (11.6 %), iso-C17 : 1 I/anteiso-C17 : 1 B (10.1 %), iso-C17 : 0 3-OH (9.2 %) and iso-C17 : 1ω9c/C16 : 0 10-methyl (7.1 %), which accounted for 70.0 % of the total fatty acids. DNA-DNA hybridization showed that strain 12C11(T) shared low DNA-DNA relatedness with A. vanfongensis KMM 6241(T) and A. ornithinivorans IMSNU 14014(T) (30.7±0.9 and 30.5±1.8 %, respectively). The G+C content of the chromosomal DNA of strain 12C11(T) was 38.4 mol%. The major respiratory quinones were MK-7 (96.0 %) and MK-6 (4.0 %). According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, the novel strain most appropriately belongs to the genus Algoriphagus, but can readily be distinguished from known Algoriphagus species. The name Algoriphagus zhangzhouensis sp. nov. is proposed (type strain 12C11(T) = CGMCC 1.11027(T) = MCCC 1F01099(T) = DSM 25035(T)).
一株革兰氏阴性、短杆状、非运动的细菌，菌株 12C11(T)，从中国福建省浮宫红树林沉积物中富集的一个石油降解菌混合群中分离得到。最适生长温度为 25°C，pH 值为 7.5，盐度为 4%（w/v）。比较 16S rRNA 基因序列分析表明，它与 Algoriphagus 属的成员具有最高的相似性（97.5-93.4%），与 Algoriphagus ornithinivorans IMSNU 14014(T)的序列相似性为 97.5%，其次是 Algoriphagus vanfongensis KMM 6241(T)（97.2%）；与属内其他成员的序列相似性<96.0%。菌株 12C11(T)与 A. vanfongensis KMM 6241(T)、A. ornithinivorans IMSNU 14014(T)、Algoriphagus marincola SW-2(T)和 Algoriphagus hitonicola 7-UAH(T)的 gyrB（DNA 回旋酶亚基 B）基因序列相似性分别为 78.8、78.6、75.6 和 77.4%。基于这些看家基因的系统发育树表明，菌株 12C11(T)和其他 Algoriphagus 菌株形成了一个独特的谱系。优势脂肪酸为 iso-C15：0（32.1%）、C16：1ω7c/C16：1ω6c（11.6%）、iso-C17：1 I/anteiso-C17：1 B（10.1%）、iso-C17：0 3-OH（9.2%）和 iso-C17：1ω9c/C16：0 10-methyl（7.1%），占总脂肪酸的 70.0%。DNA-DNA 杂交表明，菌株 12C11(T)与 A. vanfongensis KMM 6241(T)和 A. ornithinivorans IMSNU 14014(T)的 DNA-DNA 相关性较低（分别为 30.7±0.9 和 30.5±1.8%）。菌株 12C11(T)的染色体 DNA 的 G+C 含量为 38.4mol%。主要呼吸醌为 MK-7（96.0%）和 MK-6（4.0%）。根据其形态、生理、脂肪酸组成和 16S rRNA 基因序列数据，该新菌株最适合归入 Algoriphagus 属，但可与已知的 Algoriphagus 种明显区分开来。提议将其命名为 Algoriphagus zhangzhouensis sp. nov.（模式菌株 12C11(T) = CGMCC 1.11027(T) = MCCC 1F01099(T) = DSM 25035(T)）。
