Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences and State Key Laboratory for Marine Environmental Sciences, Xiamen University, Xiamen 361005, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2320-2325. doi: 10.1099/ijs.0.040881-0. Epub 2012 Nov 16.
A Gram-staining-negative, rod-shaped and non-motile bacterial strain, designated 12C25(T), was isolated from the crude-oil-degrading bacterial consortium enriched from mangrove sediment collected in Fujian Province, China. Optimal growth was observed at 25-28 °C, at pH 7.0 and in the presence of 2% (w/v) NaCl + 2% (w/v) KCl. Comparative 16S rRNA gene sequence analysis demonstrated that strain 12C25(T) shared the highest sequence similarity with members of the genus Muricauda (97.7-93.9%), exhibiting 97.7% sequence similarity and 33.7 ± 4% DNA-DNA relatedness to Muricauda aquimarina SW-63(T). The DNA G+C content of strain 12C25(T) was 39.9 mol%. The dominant fatty acids were iso-C15:1 G, iso-C17:0 3-OH, iso-C15:0, C18:0 and iso-C15:0 3-OH, and menaquinone with six isoprene units (MK-6) was the only respiratory quinone. On the basis of phenotypic data and phylogenetic inference, the novel strain belongs to the genus Muricauda, but can readily be distinguished from known species of this genus http://dx.doi.org/10.1601/nm.8170 and thus represents a novel species of the genus Muricauda. The name Muricauda zhangzhouensis sp. nov. is proposed and the type strain is 12C25(T) (=CGMCC 1.11028(T)=MCCC 1F01096(T)=DSM 25030(T)).
一株革兰氏阴性、杆状、不运动的细菌菌株，被命名为 12C25(T)，是从中国福建省红树林沉积物中富集的原油降解细菌群落中分离得到的。最佳生长条件为 25-28°C、pH 值 7.0 和 2%（w/v）NaCl+2%（w/v）KCl。比较 16S rRNA 基因序列分析表明，菌株 12C25(T)与 Muricauda 属的成员具有最高的序列相似性（97.7-93.9%），与 Muricauda aquimarina SW-63(T)的序列相似性为 97.7%，DNA-DNA 同源性为 33.7±4%。菌株 12C25(T)的 DNA G+C 含量为 39.9 mol%。主要脂肪酸为 iso-C15:1 G、iso-C17:0 3-OH、iso-C15:0、C18:0 和 iso-C15:0 3-OH，唯一的呼吸醌为menaquinone with six isoprene units (MK-6)。基于表型数据和系统发育推断，该新菌株属于 Muricauda 属，但与该属已知种易于区分，因此代表 Muricauda 属的一个新种。命名为 Muricauda zhangzhouensis sp. nov.，其模式菌株为 12C25(T)=CGMCC 1.11028(T)=MCCC 1F01096(T)=DSM 25030(T)。
