Ren Hugang, Du Congwu, Park Kicheon, Volkow Nora D, Pan Yingtian
Appl Phys Lett. 2012 Jun 4;100(23):233702-2337024. doi: 10.1063/1.4726115. Epub 2012 Jun 5.
We present particle counting ultrahigh-resolution optical Doppler tomography (pc-μODT) that enables accurate imaging of red blood cell velocities (ν(RBC)) of cerebrovascular networks by detecting the Doppler phase transients induced by the passage of a RBC through a capillary. We apply pc-μODT to image the response of capillary ν(RBC) to mild hypercapnia in mouse cortex. The results show that ν(RBC) in normocapnia (ν(N) = 0.72 ± 0.15 mm/s) increased 36.1% ± 5.3% (ν(H) = 0.98 ± 0.29 mm/s) in response to hypercapnia. Due to uncorrected angle effect and low hematocrit (e.g., ∼10%), ν(RBC) directly measured by μODT were markedly underestimated (ν(N) ≈ 0.27 ± 0.03 mm/s, ν(H) ≈ 0.37± 0.05 mm/s). Nevertheless, the measured ν(RBC) increase (35.3%) matched that (36.1% ± 5.3%) by pc-μODT.
我们展示了粒子计数超高分辨率光学多普勒断层扫描技术（pc-μODT），该技术通过检测红细胞（RBC）通过毛细血管时引起的多普勒相位瞬变，能够对脑血管网络中的红细胞速度（ν(RBC)）进行精确成像。我们应用pc-μODT对小鼠皮层中毛细血管ν(RBC)对轻度高碳酸血症的反应进行成像。结果表明，在正常碳酸血症中（ν(N) = 0.72 ± 0.15毫米/秒），ν(RBC)在高碳酸血症反应中增加了36.1% ± 5.3%（ν(H) = 0.98 ± 0.29毫米/秒）。由于未校正的角度效应和低血细胞比容（例如，约10%），通过μODT直接测量的ν(RBC)被明显低估（ν(N) ≈ 0.27 ± 0.03毫米/秒，ν(H) ≈ 0.37 ± 0.05毫米/秒）。然而，测量到的ν(RBC)增加（35.3%）与通过pc-μODT测量的增加（36.1% ± 5.3%）相匹配。
