巴西日圆线虫的发育脂质组

The developmental lipidome of Nippostrongylus brasiliensis.

作者信息

Wang Tao, Leeming Michael G, Williamson Nicholas A, Bouchery Tiffany, Doolan Rory, Le Gros Graham, Reid Gavin E, Harris Nicola L, Gasser Robin B

机构信息

Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, VIC, 3010, Australia.

Bio21 Mass Spectrometry and Proteomics Facility, The University of Melbourne, Parkville, VIC, 3010, Australia.

出版信息

Parasit Vectors. 2025 Jan 25;18(1):27. doi: 10.1186/s13071-024-06654-2.

DOI:10.1186/s13071-024-06654-2

PMID:39863914

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11762861/

Abstract

BACKGROUND

Nippostrongylus brasiliensis-a nematode of rodents-is commonly used as a model to study the immunobiology of parasitic nematodes. It is a member of the Strongylida-a large order of socioeconomically important parasitic nematodes of animals. Lipids are known to play essential roles in nematode biology, influencing cellular membranes, energy storage and/or signalling.

METHODS

The present investigation provides a comprehensive, untargeted lipidomic analysis of four developmental stages/sexes (i.e. egg, L3, adult female and adult male stages) of N. brasiliensis utilising liquid chromatography coupled to mass spectrometry.

RESULTS

We identified 464 lipid species representing 18 lipid classes and revealed distinct stage-specific changes in lipid composition throughout nematode development. Triacylglycerols (TGs) dominated the lipid profile in the egg stage, suggesting a key role for them in energy storage at this early developmental stage. As N. brasiliensis develops, there was a conspicuous transition toward membrane-associated lipids, including glycerophospholipids (e.g. PE and PC) and ether-linked lipids, particularly in adult stages, indicating a shift toward host adaptation and membrane stabilisation.

CONCLUSIONS

We provide a comprehensive insight into the lipid composition and abundance of key free-living and parasitic stages of N. brasiliensis. This study provides lipidomic resources to underpin the detailed exploration of lipid biology in this model parasitic nematode.

摘要

背景

巴西日圆线虫——一种啮齿动物的线虫——通常被用作研究寄生线虫免疫生物学的模型。它是圆线目（Strongylida）的成员之一，圆线目是一类对社会经济具有重要意义的动物寄生线虫。已知脂质在 nematode 生物学中发挥着重要作用，影响细胞膜、能量储存和/或信号传导。

方法

本研究利用液相色谱-质谱联用技术，对巴西日圆线虫的四个发育阶段/性别（即卵、L3、成年雌性和成年雄性阶段）进行了全面的非靶向脂质组学分析。

结果

我们鉴定出了代表 18 种脂质类别的 464 种脂质物种，并揭示了线虫发育过程中脂质组成的明显阶段特异性变化。三酰甘油（TGs）在卵阶段的脂质谱中占主导地位，表明它们在这个早期发育阶段的能量储存中起关键作用。随着巴西日圆线虫的发育，向膜相关脂质发生了明显转变，包括甘油磷脂（如 PE 和 PC）和醚连接脂质，特别是在成年阶段，这表明向宿主适应和膜稳定的转变。