Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, United States.
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, United States.
Anal Chem. 2023 Jul 18;95(28):10603-10609. doi: 10.1021/acs.analchem.3c00753. Epub 2023 Jul 7.
Lipids are essential macromolecules that play a crucial role in numerous biological events. Lipids are structurally diverse which allows them to fulfill multiple functional roles. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful tool to understand the spatial localization of lipids within biological systems. Herein, we report the use of ammonium fluoride (NHF) as a comatrix additive to enhance lipid detection in biological samples, with a signal increase of up to 200%. Emphasis was placed on anionic lipid enhancement with negative polarity measurements, with some preliminary work on cationic lipids detailed. We observed lipid signal enhancement of [M-H] ions with the addition of NHF additive attributed to a proton transfer reaction in several different lipid classes. Overall, our study demonstrates that the use of the NHF comatrix additive substantially improves sensitivity for lipid detection in a MALDI system and is capable of being applied to a variety of different applications.
脂质是必不可少的生物大分子，在许多生物事件中发挥着关键作用。脂质结构多样，这使它们能够发挥多种功能。基质辅助激光解吸/电离质谱成像（MALDI-MSI）是一种了解生物系统中脂质空间定位的强大工具。在这里，我们报告了使用氟化铵（NHF）作为共基质添加剂来增强生物样本中脂质的检测，信号增强高达 200%。重点放在带负电荷的阴离子脂质的增强上，并对阳离子脂质进行了一些初步的工作。我们观察到添加 NHF 添加剂后 [M-H] 离子的脂质信号增强，这归因于几种不同脂质类别的质子转移反应。总的来说，我们的研究表明，使用 NHF 共基质添加剂可以显著提高 MALDI 系统中脂质检测的灵敏度，并能够应用于各种不同的应用。
