Guangdong Provincial Key Laboratory of Food, Nutrition, and Health, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou, Guangdong Province, PR China; Department of Maternal and Child Health, School of Public Health, Sun Yat-Sen University, Guangzhou, Guangdong Province, PR China.
Nutr Metab Cardiovasc Dis. 2013 Sep;23(9):843-9. doi: 10.1016/j.numecd.2012.06.005. Epub 2012 Aug 17.
Atherosclerosis is a chronic inflammatory disease and previous studies have demonstrated that anthocyanin inhibits atherosclerosis. In the present study, we explored the effects of anthocyanins on inflammatory cytokines in hypercholesterolemic adults and cell lines.
A total of 150 subjects with hypercholesterolemia consumed a purified anthocyanin mixture (320 mg/d) or a placebo twice a day for 24 weeks in a randomized, double-blind trial. Anthocyanin consumption significantly decreased the levels of serum high sensitivity C-reactive protein (hsCRP) (-21.6% vs. -2.5%, P = 0.001), soluble vascular cell adhesion molecule-1 (sVCAM-1) (-12.3% vs. 0.4%, P = 0.005) and plasma IL-1β (-12.8% vs. -1.3%, P = 0.019) compared to the placebo. We also found a significant difference in the LDL-cholesterol (-10.4% vs. 0.3%, P = 0.030) and HDL-cholesterol level changes (14.0% vs. -0.9%, P = 0.036) between the two groups. In cell culture assays in vitro, purified anthocyanin mixture, delphinidin-3-Ο-β-glucoside (Dp-3g) and cyanidin-3-Ο-β-glucoside (Cy-3g) inhibited IL-6 and IL-1β-induced CRP production (P < 0.05) in HepG2 cell line and LPS-induced VCAM-1 secretion (P < 0.05) in porcine iliac artery endothelial cell line respectively in a dose-dependent manner. In addition, the reduction of inflammatory cytokines associated with anthocyanin mixture was stronger when compared with the effects of Dp-3g and Cy-3g separately (P < 0.05).
Anthocyanin mixture reduced the inflammatory response in hypercholesterolemic subjects. In addition, different anthocyanin compounds were found to have additive or synergistic effects in mediating anti-inflammatory responses in vitro cell culture assays.
动脉粥样硬化是一种慢性炎症性疾病，先前的研究表明，花色苷可抑制动脉粥样硬化。本研究旨在探讨花色苷对高胆固醇血症成人和细胞系中炎症细胞因子的影响。
共 150 名高胆固醇血症患者参与了一项随机、双盲临床试验，他们每天两次服用纯化花色苷混合物（320mg/d）或安慰剂，持续 24 周。与安慰剂相比，花色苷的摄入显著降低了血清高敏 C 反应蛋白（hsCRP）（-21.6%对-2.5%，P=0.001）、可溶性血管细胞黏附分子-1（sVCAM-1）（-12.3%对 0.4%，P=0.005）和血浆白细胞介素-1β（IL-1β）（-12.8%对-1.3%，P=0.019）的水平。我们还发现两组间 LDL-胆固醇（-10.4%对 0.3%，P=0.030）和 HDL-胆固醇水平变化（14.0%对-0.9%，P=0.036）存在显著差异。在体外细胞培养实验中，纯化的花色苷混合物、矢车菊素-3-O-β-葡萄糖苷（Dp-3g）和飞燕草素-3-O-β-葡萄糖苷（Cy-3g）均能抑制 HepG2 细胞系中 IL-6 和 IL-1β诱导的 CRP 产生（P<0.05），以及猪髂动脉内皮细胞系中 LPS 诱导的 VCAM-1 分泌（P<0.05），且呈剂量依赖性。此外，与单独使用 Dp-3g 和 Cy-3g 相比，花色苷混合物降低炎症细胞因子的作用更强（P<0.05）。
花色苷混合物可降低高胆固醇血症患者的炎症反应。此外，不同的花色苷化合物在体外细胞培养实验中具有协同或相加的抗炎作用。
