Arisue Atsuhiro, Shimojima Naoki, Tomiya Masayuki, Shimizu Takayuki, Harada Daisuke, Nakayama Mitsuo, Tomita Hirofumi, Shinoda Masahiro, Tanabe Minoru, Maruyama Ikuro, Mizuno Masaru, Kuroda Tatsuo, Wakabayashi Go, Morikawa Yasuhide
School of Medicine, Department of Pediatric Surgery, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Pediatr Surg Int. 2012 Sep;28(9):913-8. doi: 10.1007/s00383-012-3144-0.
The usefulness of omega-3 lipid emulsions has been extensively studied. The objectives of the present study were to examine the effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia-reperfusion injury and the underlying mechanism.
A total of 66 rats were divided into three dietary groups (lipid-free, soybean oil, and fish oil groups). Each animal was administered total parenteral nutrition for 3 days, followed by induction of intestinal ischemia for 100 min. Animals subjected to sham surgery served as the controls. Intestinal tissue and blood were harvested 6 and 12 h after the surgery, then, assessment of the histological damage score, plasma-related parameters, and statistical evaluation were performed.
The histological damage score in the intestinal tissues was significantly lower in the fish oil group than in the soybean oil group (P = 0.0121). The late-phase urinary level of 8-hydroxy-2-deoxyguanosine was also significantly lower in the fish oil group as compared with that in the other groups (P = 0.0267). Furthermore, the plasma level of high-mobility group box 1 protein was also significantly lower in the fish oil group as compared with that in the lipid-free group (P = 0.0398).
It appeared that intravenous administration of an omega-3 lipid emulsion prior to ischemia-reperfusion injury reduced the oxidative stress and severity of tissue damage. Modification of membrane fatty acids may serve as the mechanism underlying this reduction of tissue damage.
ω-3 脂质乳剂的效用已得到广泛研究。本研究的目的是检验ω-3 脂质乳剂在减轻大鼠肠缺血再灌注损伤模型中的氧化应激作用及其潜在机制。
总共 66 只大鼠被分为三个饮食组（无脂组、大豆油组和鱼油组）。每只动物接受 3 天的全胃肠外营养，随后诱导肠缺血 100 分钟。接受假手术的动物作为对照。术后 6 小时和 12 小时采集肠组织和血液，然后进行组织学损伤评分、血浆相关参数评估及统计学评价。
鱼油组肠组织的组织学损伤评分显著低于大豆油组（P = 0.0121）。与其他组相比，鱼油组晚期尿中 8-羟基-2-脱氧鸟苷水平也显著降低（P = 0.0267）。此外，与无脂组相比，鱼油组血浆中高迁移率族蛋白 B1 的水平也显著降低（P = 0.0398）。
似乎在缺血再灌注损伤前静脉注射ω-3 脂质乳剂可减轻氧化应激和组织损伤的严重程度。膜脂肪酸的改变可能是这种组织损伤减轻的潜在机制。
