Division of Energy Systems Research, Ajou University, Suwon, 443-749 Korea.
Chemistry. 2012 Sep 24;18(39):12388-94. doi: 10.1002/chem.201201197. Epub 2012 Aug 21.
Two-photon microscopy (TPM) has become an indispensable tool in the study of biology and medicine due to the capability of this method for molecular imaging deep inside intact tissues. For the maximum utilization of TPM, a variety of two-photon (TP) probes for specific applications are needed. In this article, we report a small-molecule TP probe (ANO1) for nitric oxide (NO) that shows a rapid and specific NO response, a 68-fold fluorescence enhancement in response to NO, and a maximum TP-action cross-section of 170 GM (GM: 10(-50) cm(4) photon(-1)) upon reaction with excess NO. This probe can be easily loaded into cells and tissues and can real-time monitor NO in living tissues at 100-180 μm depth for longer than 1200 s through the use of TPM, with minimum interference from other biologically relevant species.
双光子显微镜（TPM）已经成为生物学和医学研究中不可或缺的工具，因为该方法能够对完整组织内的分子进行成像。为了最大程度地利用 TPM，需要各种用于特定应用的双光子（TP）探针。在本文中，我们报告了一种用于一氧化氮（NO）的小分子 TP 探针（ANO1），该探针对 NO 具有快速和特异性的响应，NO 响应的荧光增强 68 倍，与过量 NO 反应时的最大 TP 作用截面为 170 GM（GM：10(-50) cm(4) photon(-1))。该探针可以很容易地载入细胞和组织中，并可以通过 TPM 在 100-180 μm 的深度实时监测活组织中的 NO，时间超过 1200 s，并且受到其他生物学相关物质的最小干扰。
