Sarkar Avik Ranjan, Kang Dong Eun, Kim Hwan Myung, Cho Bong Rae
Division of Energy Systems Research, Ajou University , Suwon 443-749, Korea.
Inorg Chem. 2014 Feb 17;53(4):1794-803. doi: 10.1021/ic402475f. Epub 2013 Dec 12.
Two-photon microscopy (TPM) is a new imaging tool that can detect biological targets deep inside a live tissue. To faciltate the use of TPM in biomedical research, a variety of two-photon (TP) probes for specific applications are needed. In this Forum Article, we describe the design strategy, photophysical properties, and biological imaging applications of a selection of our recent studies in the development of TP probes for metal ions. Small-molecule TP turn-on probes, organelle-targeted probes, and multicolor emissive probes for dual-color imaging are briefly reviewed.
双光子显微镜（TPM）是一种新型成像工具，能够检测活组织内部深处的生物靶点。为促进TPM在生物医学研究中的应用，需要各种用于特定应用的双光子（TP）探针。在这篇论坛文章中，我们描述了我们最近在开发金属离子TP探针方面的一系列研究的设计策略、光物理性质和生物成像应用。简要综述了用于双色成像的小分子TP开启探针、细胞器靶向探针和多色发射探针。
