Department of Chemistry, Korea University, Seoul 136-701, Korea.
BMB Rep. 2013 Apr;46(4):188-94. doi: 10.5483/bmbrep.2013.46.4.045.
Two-photon microscopy (TPM), which uses two photons of lower energy as the excitation source, is a vital tool in biology and clinical science, due to its capacity to image deep inside intact tissues for a long period of time. To make TPM a more versatile tool in biomedical research, we have developed a variety of two-photon probes for specific applications. In this mini review, we will briefly discuss two-photon probes for lipid rafts, lysosomes, mitochondria, and pH, and their biomedical applications.
双光子显微镜（TPM）使用两个低能量的光子作为激发源，由于其能够在长时间内对完整组织内部进行深层成像，因此成为生物学和临床科学的重要工具。为了使 TPM 成为生物医学研究中更通用的工具，我们已经开发了各种用于特定应用的双光子探针。在这个迷你综述中，我们将简要讨论用于脂筏、溶酶体、线粒体和 pH 的双光子探针及其在生物医学中的应用。
