Ho Chen-Lung, Hua Kuo-Feng, Hsu Kuan-Ping, Wang Eugene I-chen, Su Yu-Chang
Division of Wood Cellulose, Taiwan Forestry Research Institute, Taipei, Taiwan 100.
Nat Prod Commun. 2012 Jul;7(7):933-6.
In this study, antipathogenic activities of the twig essential oil and its constituents from Chamaecyparis formosensis Matsum were evaluated in vitro against six plant pathogenic fungi. The essential oil from the fresh twigs was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Twenty-five compounds were identified, representing 98.9% of the oil. The main components were beta-eudesmol (25.1%), tau-muurolol (21.6%), elemol (15.0%), totarol (14.9%), and alpha-cadinol (12.4%). The twig oil (500 mcirog/mL) showed growth inhibitory activity against the phytopathogenic fungi, Fusarium oxysporum, Pestalotiopsis funereal, and Ganoderma austral, with antifungal indices of 92.7%, 71.1%, and 87.7%, respectively. In addition, the oil suppressed totally the growth of Rhizoctonia solani, Colletotrichum gloeosporioides, and Fusarium solani. In order to ascertain the source compounds of these antipathogenic activities, the main components were individually evaluated. Tau-Muurolol and alpha-cadinol exhibited excellent activity against F. oxysporum, R. solani, C. gloeosporioides, and F. solani, with IC50 < 50 microg/mL. These compounds also efficiently inhibited the mycelial growths of P. funereal and G. austral. Thus, alpha-cadinol and tau-muurolol could be considered as potential natural fungicides for controlling fungal pathogens and worth.
本研究对台湾扁柏嫩枝精油及其成分进行体外抗植物病原真菌活性评价，测试其对6种植物病原真菌的抑制作用。采用Clevenger型装置通过水蒸馏法从新鲜嫩枝中分离出精油，并用气相色谱 - 氢火焰离子化检测器（GC - FID）和气相色谱 - 质谱联用仪（GC - MS）对其进行表征。共鉴定出25种化合物，占精油总量的98.9%。主要成分是β - 桉叶醇（25.1%）、τ - 穆罗醇（21.6%）、榄香醇（15.0%）、脱氢枞醇（14.9%）和α - 杜松醇（12.4%）。嫩枝精油（500微克/毫升）对植物病原真菌尖孢镰刀菌、黑盘孢菌和南方灵芝具有生长抑制活性，其抗真菌指数分别为92.7%、71.1%和87.7%。此外，该精油完全抑制了立枯丝核菌、胶孢炭疽菌和茄类镰刀菌的生长。为确定这些抗病原活性的来源化合物，对主要成分分别进行了评估。τ - 穆罗醇和α - 杜松醇对尖孢镰刀菌、立枯丝核菌、胶孢炭疽菌和茄类镰刀菌表现出优异的活性，半数抑制浓度（IC50）< 50微克/毫升。这些化合物还能有效抑制黑盘孢菌和南方灵芝的菌丝生长。因此，α - 杜松醇和τ - 穆罗醇可被视为控制真菌病原体的潜在天然杀菌剂，具有研究价值。
