Spine Research Group, Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland.
J Inflamm (Lond). 2012 Aug 21;9(1):29. doi: 10.1186/1476-9255-9-29.
As proinflammatory cytokines seem to play a role in discogenic back pain, substances exhibiting anti-inflammatory effects on intervertebral disc cells may be used as minimal-invasive therapeutics for intradiscal/epidural injection. The purpose of this study was to investigate the anti-inflammatory and anti-catabolic potential of curcuma, which has been used in the Indian Ayurvedic medicine to treat multiple ailments for a long time.
Human disc cells were treated with IL-1β to induce an inflammatory/catabolic cascade. Different extracts of curcuma as well as curcumin (= a component selected based on results with curcuma extracts and HPLC/MS analysis) were tested for their ability to reduce mRNA expression of proinflammatory cytokines and matrix degrading enzymes after 6 hours (real-time RT-PCR), followed by analysis of typical inflammatory signaling mechanisms such as NF-κB (Western Blot, Transcription Factor Assay), MAP kinases (Western Blot) and Toll-like receptors (real-time RT-PCR). Quantitative data was statistically analyzed using a Mann Whitney U test with a significance level of p < 0.05 (two-tailed).
Results indicate that the curcuma DMSO extract significantly reduced levels of IL-6, MMP1, MMP3 and MMP13. The DMSO-soluble component curcumin, whose occurrence within the DMSO extract was verified by HPLC/MS, reduced levels of IL-1β, IL-6, IL-8, MMP1, MMP3 and MMP13 and both caused an up-regulation of TNF-α. Pathway analysis indicated that curcumin did not show involvement of NF-κB, but down-regulated TLR2 expression and inhibited the MAP kinase JNK while activating p38 and ERK.
Based on its anti-inflammatory and anti-catabolic effects, intradiscal injection of curcumin may be an attractive treatment alternative. However, whether the anti-inflammatory properties in vitro lead to analgesia in vivo will need to be confirmed in an appropriate animal model.
由于促炎细胞因子似乎在椎间盘源性腰痛中发挥作用，因此具有抗椎间盘细胞炎症作用的物质可能被用作椎间盘内/硬膜外注射的微创治疗方法。本研究的目的是研究姜黄的抗炎和抗分解代谢作用，姜黄长期以来一直被用于印度阿育吠陀医学来治疗多种疾病。
用白细胞介素-1β（IL-1β）处理人椎间盘细胞，以诱导炎症/分解代谢级联反应。测试了姜黄的不同提取物以及姜黄素（=根据姜黄提取物和 HPLC/MS 分析的结果选择的一种成分），以检测它们在 6 小时后降低促炎细胞因子和基质降解酶的 mRNA 表达的能力（实时 RT-PCR），然后分析典型的炎症信号机制，如 NF-κB（Western Blot、转录因子测定）、MAP 激酶（Western Blot）和 Toll 样受体（实时 RT-PCR）。使用具有双侧检验的 Mann-Whitney U 检验对定量数据进行统计分析，显著性水平为 p<0.05。
结果表明，姜黄 DMSO 提取物显著降低了 IL-6、MMP1、MMP3 和 MMP13 的水平。DMSO 可溶性成分姜黄素，其在 DMSO 提取物中的存在通过 HPLC/MS 得到证实，降低了 IL-1β、IL-6、IL-8、MMP1、MMP3 和 MMP13 的水平，同时导致 TNF-α 的上调。通路分析表明，姜黄素不参与 NF-κB，但下调 TLR2 表达并抑制 MAP 激酶 JNK，同时激活 p38 和 ERK。
基于其抗炎和抗分解代谢作用，姜黄素的椎间盘内注射可能是一种有吸引力的治疗选择。然而，体外的抗炎特性是否会导致体内的镇痛作用，需要在适当的动物模型中得到证实。
