Department of Forensic Science, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Forensic Sci Int. 2012 Oct 10;222(1-3):313-7. doi: 10.1016/j.forsciint.2012.07.008. Epub 2012 Aug 19.
Superwarfarin poisoning is a growing health problem. A sensitive and reproducible LC-ESI/MS/MS (liquid chromatography electrospray ionization tandem mass spectrometry) method was developed and validated for the determination of bromadiolone and brodifacoum, the most commonly used superwarfarins, in human blood using warfarin-D5 as an internal standard. Bromadiolone and brodifacoum were extracted from whole blood samples by liquid-liquid extraction with ethyl acetate. Multiple-reaction monitoring (MRM) was used to detect bromadiolone and brodifacoum using precursor→product ion combinations of m/z 525→250 and 521→135, respectively. The calibration curves were linear (r(2)=0.9999) in the concentration range of 0.5-100.0 ng/mL for bromadiolone and brodifacoum, with a lower limit of detection of 0.1 and 0.2 ng/mL, respectively, in whole blood. This method detected trace levels of bromadiolone and brodifacoum in whole blood samples and can be used in the diagnosis of poisoned human beings.
超级杀鼠剂中毒是一个日益严重的健康问题。本研究开发并验证了一种灵敏且重现性良好的 LC-ESI/MS/MS（液相色谱-电喷雾电离串联质谱）方法，用于检测人血中溴敌隆和溴鼠灵（最常用的超级杀鼠剂），检测时以华法林-D5 作为内标。溴敌隆和溴鼠灵采用乙酸乙酯液液萃取法从全血样品中提取。采用多反应监测（MRM）模式，通过 m/z 525→250 和 521→135 分别检测溴敌隆和溴鼠灵的前体→产物离子对。溴敌隆和溴鼠灵的校准曲线在 0.5-100.0ng/mL 浓度范围内呈线性（r(2)=0.9999），在全血中的检测限分别为 0.1 和 0.2ng/mL。该方法能够检测全血样本中痕量的溴敌隆和溴鼠灵，可用于诊断中毒患者。
