Diabetes Research Group, Division of Diabetes and Nutritional Sciences, School of Medicine, King's College London, London SE1 1UL, UK.
Phytother Res. 2013 Jul;27(7):1006-11. doi: 10.1002/ptr.4815. Epub 2012 Aug 21.
Herbal medicines, especially plant-derived extracts, have been used to treat Type 2 diabetes mellitus (T2DM) for many centuries, and offer the potential of cheap and readily available alternatives to conventional pharmaceuticals in developing countries. Extracts of Gymnema sylvestre (GS) have anti-diabetic activities and have been used as a folk medicine in India for centuries. We have investigated the effects of a novel high molecular weight GS extract termed OSA® on glucose tolerance in insulin-resistant ob/ob mice, and on insulin secretion and synthesis by isolated mouse islets. Single administration of OSA® (500 mg/kg) to ob/ob mice 30 min before an intraperitoneal glucose load improved their abnormal glucose tolerance. In vitro studies indicated that OSA® (0.25 mg/ml) initiated rapid and reversible increases in insulin secretion from isolated mouse islets at substimulatory (2 mM) and stimulatory (20 mM) glucose concentrations. In addition, prolonged treatment (24-48 h) of mouse islets with OSA® elevated the expression of preproinsulin mRNA and maintained the total insulin content of mouse islets in the presence of stimulated insulin secretion. These effects of OSA® are consistent with its potential use as a therapy for the hyperglycemia associated with obesity-related T2DM.
草药，特别是植物提取物，已被用于治疗 2 型糖尿病（T2DM）数百年，并且为发展中国家提供了传统药物的廉价且易于获得的替代品的潜力。武靴藤（GS）的提取物具有抗糖尿病作用，并且在印度已经被用作民间药物数百年。我们研究了一种新型高分子量 GS 提取物 OSA®对胰岛素抵抗 ob/ob 小鼠葡萄糖耐量的影响，以及对分离的小鼠胰岛胰岛素分泌和合成的影响。OSA®（500mg/kg）在腹腔内葡萄糖负荷前 30 分钟单次给药可改善 ob/ob 小鼠的异常葡萄糖耐量。体外研究表明，OSA®（0.25mg/ml）在亚刺激（2mM）和刺激（20mM）葡萄糖浓度下，迅速和可逆地增加分离的小鼠胰岛的胰岛素分泌。此外，OSA®（24-48 小时）处理小鼠胰岛可增加前胰岛素 mRNA 的表达，并在刺激胰岛素分泌的情况下维持小鼠胰岛的总胰岛素含量。OSA®的这些作用与其作为肥胖相关 T2DM 相关高血糖症治疗的潜在用途一致。
