Parveen Shabana, Ansari Mohd Hafizur Rehman, Parveen Rabea, Khan Washim, Ahmad Sayeed, Husain Syed Akhtar
Human Genetics Laboratory, Department of Bioscience, Jamia Millia Islamia, New Delhi 110025, India.
Bioactive Natural Product Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
Evid Based Complement Alternat Med. 2019 Jan 6;2019:7523159. doi: 10.1155/2019/7523159. eCollection 2019.
, popularly known as gurmar, is extensively used in traditional systems of medicine for diabetes, stomach ailments, liver diseases, and cardiac disorders. Dried leaf powder of was extracted through soxhlation using 70% (v/v) alcohol. The hydroalcoholic extract was concentrated to 1/4th of its volume and basified to isolate gymnemic acid enriched extract using chloroform. The isolated extract was checked for its antioxidant potential against 1, 1-diphenyl-2-picryl-hydrazyl (DPPH), which showed scavenging activity of 82.31% at 80 g/mL of extract. Quality control analysis of the extract was carried out by TLC. Chloroform and methanol (9.5:0.5, v/v) were used as a solvent system and separated compounds were detected at 254 and 366 nm. A total of 13 metabolites were separated. However, major peaks were at R 0.12, 0.69, 0.79, and 0.85. Further, UPLC-MS fingerprinting of the extract was done using acetonitrile and 0.5% formic acid in water as mobile phase in gradient elution mode. A total of 21 metabolites were separated and tentatively identified from the database. Deacyl gymnemic acid and quercetin are the two major metabolites found in the extract. Gymnemic acid, deacyl gymnemic acid, and quercetin were docked with ten different proteins associated with glucose metabolism, transport, and glucose utilization. It has been observed that gymnemic acid was more potent than deacyl gymnemic acid in terms of binding affinity towards proteins and showed a favorable interaction with amino acid residues at the active site. Thus, the present study gives an insight of identified metabolites with protein interaction and a reason for the hypoglycemic potential of deacyl gymnemic acid enriched extract, which can be further explored for and studies to establish its phytopharmacological and therapeutic effect.
[植物名称]，俗称匙羹藤，在传统医学体系中被广泛用于治疗糖尿病、胃部疾病、肝脏疾病和心脏疾病。[植物名称]的干叶粉末通过使用70%（v/v）乙醇的索氏提取法进行提取。水醇提取物浓缩至其体积的四分之一，并碱化以使用氯仿分离富含匙羹藤酸的提取物。对分离出的提取物进行了针对1,1-二苯基-2-苦基肼（DPPH）的抗氧化潜力检测，在提取物浓度为80μg/mL时显示出82.31%的清除活性。提取物的质量控制分析通过薄层色谱法（TLC）进行。氯仿和甲醇（9.5:0.5，v/v）用作溶剂系统，分离出的化合物在254和366nm处进行检测。总共分离出13种代谢物。然而，主要峰位于比移值0.12、0.69、0.79和0.85处。此外，提取物的超高效液相色谱-质谱联用（UPLC-MS）指纹图谱分析采用乙腈和0.5%甲酸水溶液作为流动相，以梯度洗脱模式进行。总共分离出21种代谢物，并根据数据库进行了初步鉴定。去酰基匙羹藤酸和槲皮素是提取物中发现的两种主要代谢物。匙羹藤酸、去酰基匙羹藤酸和槲皮素与十种与葡萄糖代谢、转运和葡萄糖利用相关的不同蛋白质进行了对接。据观察，就对蛋白质的结合亲和力而言，匙羹藤酸比去酰基匙羹藤酸更有效，并且在活性位点与氨基酸残基表现出良好的相互作用。因此，本研究揭示了已鉴定代谢物与蛋白质相互作用的情况以及富含去酰基匙羹藤酸提取物具有降血糖潜力的原因，这可进一步用于[研究方向1]和[研究方向2]研究，以确立其植物药理学和治疗效果。
