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果蝇 MI-2 染色质重塑因子调节体内的高级染色质结构和黏连蛋白动力学。

The Drosophila MI-2 chromatin-remodeling factor regulates higher-order chromatin structure and cohesin dynamics in vivo.

机构信息

Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.

出版信息

PLoS Genet. 2012;8(8):e1002878. doi: 10.1371/journal.pgen.1002878. Epub 2012 Aug 9.

DOI:10.1371/journal.pgen.1002878
PMID:22912596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3415455/
Abstract

dMi-2 is a highly conserved ATP-dependent chromatin-remodeling factor that regulates transcription and cell fates by altering the structure or positioning of nucleosomes. Here we report an unanticipated role for dMi-2 in the regulation of higher-order chromatin structure in Drosophila. Loss of dMi-2 function causes salivary gland polytene chromosomes to lose their characteristic banding pattern and appear more condensed than normal. Conversely, increased expression of dMi-2 triggers decondensation of polytene chromosomes accompanied by a significant increase in nuclear volume; this effect is relatively rapid and is dependent on the ATPase activity of dMi-2. Live analysis revealed that dMi-2 disrupts interactions between the aligned chromatids of salivary gland polytene chromosomes. dMi-2 and the cohesin complex are enriched at sites of active transcription; fluorescence-recovery after photobleaching (FRAP) assays showed that dMi-2 decreases stable association of cohesin with polytene chromosomes. These findings demonstrate that dMi-2 is an important regulator of both chromosome condensation and cohesin binding in interphase cells.

摘要

dMi-2 是一种高度保守的 ATP 依赖的染色质重塑因子，通过改变核小体的结构或定位来调节转录和细胞命运。在这里，我们报告了 dMi-2 在调控果蝇中高级染色质结构中的一个意外作用。dMi-2 功能的丧失导致唾液腺多线染色体失去其特征性带型，并且比正常情况下更浓缩。相反，dMi-2 的表达增加会引发多线染色体的去浓缩，同时核体积显著增加；这种效应相对较快，并且依赖于 dMi-2 的 ATP 酶活性。实时分析显示，dMi-2 破坏了唾液腺多线染色体中对齐的染色单体之间的相互作用。dMi-2 和黏合蛋白复合物在活跃转录的位点富集；荧光恢复后光漂白（FRAP）实验表明，dMi-2 降低了黏合蛋白与多线染色体的稳定结合。这些发现表明，dMi-2 是有丝分裂细胞中染色体浓缩和黏合蛋白结合的重要调节剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/1254471d1859/pgen.1002878.g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/7fe8fadfb56a/pgen.1002878.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/eaa08226d523/pgen.1002878.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/de6ac41c0159/pgen.1002878.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/1254471d1859/pgen.1002878.g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/38a0856938df/pgen.1002878.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/eaa08226d523/pgen.1002878.g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1147/3415455/1254471d1859/pgen.1002878.g011.jpg

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