积雪草对四氯化碳致大鼠睾丸损伤的保护作用。
Protective effects of Launaea procumbens on rat testis damage by CCl4.
机构信息
Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology Bannu, Bannu, KPK, Pakistan.
出版信息
Lipids Health Dis. 2012 Aug 22;11:103. doi: 10.1186/1476-511X-11-103.
BACKGROUND
Traditionally various human diseases of kidneys, hormonal imbalance and sexual diseases are treated with Launaea procumbens (L). In the present study protective effects of methanolic extract of Launaea procumbens (LPME) was evaluated against CCl4-induced oxidative damages in rat testis.
METHODS
To examine the protective effects of Launaea procumbens on testis against oxidative stress of carbon tetrachloride in male rat, 30 male albino rats were equally divided into 5 groups (6 rats). First group was given standard diet and drinking water. Second group received CCl4 3 ml/kg intraperitoneally (30% in olive oil). Third and forth were given orally 100; 200 mg/kg b.w., in 99.8% dimethyl sulphooxide (DMSO), Launaea procumbens methanolic extracts (LPME) after 48 h of CCl4 treatment twice a week and sixth group received only LPME in DMSO at a dose of 200 mg/kg b.w., for four weeks. Protective effects of Launaea procumbens were observed on sperm concentration, motility and morphology, serum reproductive hormonal level, activity of antioxidant enzymes, lipid peroxidation (TBARS) and DNA damages.
RESULTS
Results of the present study revealed that treatment of CCl4 significantly (p < 0.01) reduced sperm concentration and motility comparatively to controls. Level of testosterone, luteinizing hormone and follicle stimulating hormone, were depleted markedly (p <0.01) with treatment of CCl4. In addition, CCl4 induction in rats reduced activities of antioxidant enzymes while increased lipid peroxidation and DNA damages. Co-administration of LPME significantly (p <0.01) improved these alterations in improving of hormonal level, activities of antioxidant enzymes and lipid peroxidation near to control rats.
CONCLUSION
From the results it is suggested that Launaea procumbens methanolic extract has the ability to protect testis against oxidative damages, possibly through antioxidant effects of its bioactive compounds.
背景
传统上，各种肾脏疾病、激素失衡和性疾病都用 Launaea procumbens（L）治疗。在本研究中，评估了 Launaea procumbens 的甲醇提取物（LPME）对大鼠睾丸 CCl4 诱导的氧化损伤的保护作用。
方法
为了研究 Launaea procumbens 对雄性大鼠睾丸对抗四氯化碳氧化应激的保护作用，将 30 只雄性白化大鼠等分为 5 组（每组 6 只）。第一组给予标准饮食和饮用水。第二组腹腔注射 3ml/kg CCl4（30%在橄榄油中）。第三组和第四组分别给予 100；200mg/kg b.w.，在 99.8%二甲基亚砜（DMSO）中口服 Launaea procumbens 甲醇提取物（LPME），在 CCl4 处理后 48 小时内每周两次，第六组仅在 DMSO 中给予 200mg/kg b.w.的 LPME，四周。观察 Launaea procumbens 对精子浓度、活力和形态、血清生殖激素水平、抗氧化酶活性、脂质过氧化（TBARS）和 DNA 损伤的保护作用。
结果
本研究结果表明，CCl4 处理显著（p <0.01）降低了精子浓度和活力与对照组相比。用 CCl4 处理后，睾酮、促黄体生成素和促卵泡激素水平明显下降（p <0.01）。此外，CCl4 诱导大鼠的抗氧化酶活性降低，脂质过氧化和 DNA 损伤增加。LPME 联合治疗显著（p <0.01）改善了这些改变，提高了激素水平、抗氧化酶活性和脂质过氧化接近对照组大鼠。
结论
从结果来看，Launaea procumbens 甲醇提取物具有保护睾丸免受氧化损伤的能力，可能通过其生物活性化合物的抗氧化作用。
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