茵陈蒿氯仿提取物对四氯化碳致大鼠肝损伤的保护作用。
Hepatoprotection with a chloroform extract of Launaea procumbens against CCl4-induced injuries in rats.
机构信息
Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology Bannu KPK, Bannu, Pakistan.
出版信息
BMC Complement Altern Med. 2012 Aug 3;12:114. doi: 10.1186/1472-6882-12-114.
BACKGROUND
Launaea procumbens (Asteraceae) is used as a folk medicine to treat hepatic disorders in Pakistan. The effect of a chloroform extract of Launaea procumbens (LPCE) was evaluated against carbon-tetrachloride (CCl4)-induced liver damage in rats.
METHODS
To evaluate the hepatoprotective effects of LPCE, 36 male Sprague-Dawley rats were equally divided into six groups. Animals of group 1 (control) had free access to food and water. Group II received 3 ml/kg of CCl4 (30% in olive oil v/v) via the intraperitoneal route twice a week for 4 weeks. Group III received 1 ml of silymarin via gavage (100 mg/kg b.w.) after 48 h of CCl4 treatment whereas groups IV and V were given 1 ml of LPCE (100 and 200 mg/kg b.w., respectively) after 48 h of CCl4 treatment. Group VI received 1 ml of LPCE (200 mg/kg b.w.) twice a week for 4 weeks. The activities of the antioxidant enzymes catalase, peroxidase (POD), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), glutathione reductase (GSR), glutathione (GSH) and lipid peroxidation (thiobarbituric acid reactive substances (TBARS)) were measured in liver homogenates. DNA damage, argyrophilic nucleolar organizer regions (AgNORs) counts and histopathology were studied in liver samples. Serum was analyzed for various biochemical parameters. Phytochemical composition in LPCE was determined through high-performance liquid chromatography (HPLC).
RESULTS
LPCE inhibited lipid peroxidation, and reduced the activities of aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate dehydrogenase in serum induced by CCl4. GSH contents were increased as were the activities of antioxidant enzymes (catalase, SOD, GST, GSR, GSH-Px) when altered due to CCl4 hepatotoxicity. Similarly, absolute liver weight, relative liver weight and the number of hepatic lesions were reduced with co-administration of LPCE. Phyochemical analyses of LPCE indicated that it contained catechin, kaempferol, rutin, hyperoside and myricetin.
CONCLUSION
These results indicated that Launaea procumbens efficiently protected against the hepatotoxicity induced by CCl4 in rats, possibly through the antioxidant effects of flavonoids present in LPCE.
背景
在巴基斯坦，蔓生千里光（菊科）被用作民间药物来治疗肝脏疾病。本研究评估了蔓生千里光（LPCE）的氯仿提取物对四氯化碳（CCl4）诱导的大鼠肝损伤的影响。
方法
为了评估 LPCE 的肝保护作用，将 36 只雄性斯普拉格-道利大鼠等分为 6 组。第 1 组（对照组）自由进食和饮水。第 2 组通过腹腔途径每周两次给予 3 ml/kg 的 CCl4（30%在橄榄油中，v/v），共 4 周。第 3 组在给予 CCl4 后 48 小时给予 1 ml 水飞蓟素灌胃（100 mg/kg b.w.），而第 4、5 组分别在给予 CCl4 后 48 小时给予 1 ml LPCE（100 和 200 mg/kg b.w.）。第 6 组每周两次给予 1 ml LPCE（200 mg/kg b.w.），共 4 周。在肝匀浆中测定抗氧化酶过氧化氢酶、过氧化物酶（POD）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽 S-转移酶（GST）、谷胱甘肽还原酶（GSR）、谷胱甘肽（GSH）和脂质过氧化（硫代巴比妥酸反应物质（TBARS））的活性。在肝组织中研究了 DNA 损伤、银染核仁组成区（AgNORs）计数和组织病理学。对血清进行了各种生化参数的分析。通过高效液相色谱法（HPLC）确定 LPCE 中的植物化学成分。
结果
LPCE 抑制了 CCl4 诱导的血清中天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶和乳酸脱氢酶的脂质过氧化作用，并降低了其活性。GSH 含量增加，同时由于 CCl4 肝毒性导致的抗氧化酶（过氧化氢酶、SOD、GST、GSR、GSH-Px）活性也增加。同样，当与 LPCE 联合使用时，绝对肝重、相对肝重和肝损伤数减少。LPCE 的植物化学成分分析表明，它含有儿茶素、山奈酚、芦丁、金丝桃苷和杨梅素。
结论
这些结果表明，蔓生千里光可有效预防 CCl4 诱导的大鼠肝毒性，可能是通过 LPCE 中存在的类黄酮的抗氧化作用。
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