Department of Biotechnology, Faculty of Sciences, University of Science and Technology Bannu, KPK, Pakistan;
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Food Nutr Res. 2016 Mar 3;60:29790. doi: 10.3402/fnr.v60.29790. eCollection 2016.
Ethyl acetate extracts of Launaea procumbens is used for the treatment of liver dysfunction as an herbal medicine in Pakistan. In this study, the protective effects of ethyl acetate extracts were evaluated against CCl4-induced liver injuries in rat.
To examine the protective effects against oxidative stress of carbon tetrachloride in rats, 30 male rats were equally divided into 5 groups (6 rats). Among five groups, one was treated with CCl4 (3 ml/kg i.p. in olive oil b.w.) twice a week for 4 weeks. Others were orally fed with extracts (100, 200 mg/kg b.w.), with CCl4 twice a week for 4 weeks.
Administration of CCl4 altered the serum marker enzymes, lipid profile, CYP 2E1, p53 expression, antioxidant enzymes, nuclear organizer regions (AgNORs), and DNA. Supplement of L. procumbens ameliorated the effects of CCl4, improved CYP 2E1, p53, and increased the activities of antioxidant enzymes while activity of liver marker enzymes (ALP, ALT, AST, g-GT) and contents of lipid per oxidation contents (TBARS), AgNORs, and DNA fragmentation were decreased. Similarly body weight was increased while liver and relative liver weight was decreased with co-administration of various extracts, suggesting that L. procumbens effectively protect liver against the CCl4-induced oxidative damage in rats.
The hepatoprotective and free radical scavenging effects might be due to the presence of bioactive constituents in the extract.
在巴基斯坦，茵陈蒿的乙酸乙酯提取物被用作治疗肝功能障碍的草药。在这项研究中，评估了乙酸乙酯提取物对大鼠 CCl4 诱导的肝损伤的保护作用。
为了研究对大鼠四氯化碳诱导的氧化应激的保护作用，将 30 只雄性大鼠等分为 5 组（6 只）。在这 5 组中，一组用 CCl4（橄榄油体重 3ml/kg，ip）每周两次处理 4 周。其余的用提取物（100、200mg/kg 体重），每周两次用 CCl4 处理 4 周。
CCl4 的给药改变了血清标志物酶、脂质谱、CYP2E1、p53 表达、抗氧化酶、核组织者区域（AgNORs）和 DNA。茵陈蒿的补充改善了 CCl4 的作用，提高了 CYP2E1、p53，并增加了抗氧化酶的活性，同时降低了肝标志物酶（碱性磷酸酶、丙氨酸转氨酶、天冬氨酸转氨酶、γ-谷氨酰转移酶）和脂质过氧化含量（TBARS）、AgNORs 和 DNA 片段化的活性。同样，体重增加，而肝脏和相对肝脏重量减少，这表明茵陈蒿能有效地保护肝脏免受 CCl4 诱导的大鼠氧化损伤。
肝保护和自由基清除作用可能是由于提取物中存在生物活性成分。
