Jiang Jinjin, Liu Yongjun, Liu Yu, Hou Siyu
Key Lab of Northwest Water Resource, Ecology and Environment, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China.
Curr Microbiol. 2018 Mar;75(3):316-322. doi: 10.1007/s00284-017-1382-z. Epub 2017 Oct 31.
A novel bacterial cells immobilized carrier (ZnONPs/PVA), polyvinyl alcohol (PVA) composites decorated with ZnO nanoparticles (ZnO NPs), was prepared and used for immobilization of the strain Ochrobactrum sp. LC-1, and subsequently for quinoline degrading in water. Characterization of ZnONPs/PVA by using X-ray diffractometer and scanning electron microscopy demonstrated that ZnO NPs were coated on the surface of PVA cubes evenly and the bacterium grew well on the ZnONPs/PVA. Quinoline biodegradation results showed that the degradation effect of quinoline by ZnONPs/PVA immobilized cells was superior to the free cells significantly. The structure and physical properties of ZnNPs/PVA were maintained steady after the reuse of ZnNPs/PVA for cells immobilization several times. Reusability of the ZnONPs/PVA immobilized cells revealed that the quinoline removal ratio was above 97% within 8 h under the conditions of pH neutral, 37 °C when the initial quinoline concentration was 300 mg/L.
制备了一种新型细菌细胞固定化载体（ZnONPs/PVA），即装饰有氧化锌纳米颗粒（ZnO NPs）的聚乙烯醇（PVA）复合材料，并将其用于固定苍白杆菌属菌株LC-1，随后用于水中喹啉的降解。利用X射线衍射仪和扫描电子显微镜对ZnONPs/PVA进行表征，结果表明ZnO NPs均匀地包覆在PVA立方体表面，且细菌在ZnONPs/PVA上生长良好。喹啉生物降解结果表明，ZnONPs/PVA固定化细胞对喹啉的降解效果明显优于游离细胞。ZnNPs/PVA用于细胞固定化多次后，其结构和物理性质保持稳定。ZnONPs/PVA固定化细胞的可重复使用性表明，当初始喹啉浓度为300 mg/L时，在pH值为中性、37℃的条件下，8小时内喹啉去除率高于97%。
