Department of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul 120-752, Republic of Korea.
J Biomed Mater Res B Appl Biomater. 2012 Oct;100(7):1935-42. doi: 10.1002/jbm.b.32760. Epub 2012 Aug 22.
In this study, we analyzed the effects of varying concentrations of chitosan (CS) and β-tricalcium phosphate [β-TCP, Ca(3)(PO(4))(2)] on the mechanical and cell-adhesion properties of a collagen (CG) matrix for use in guided bone regeneration (GBR). Three different CS concentrations (0.5, 1, and 2%) and five different contents of β-TCP (0, 17, 29, 38, and 44%) were investigated. The composite membranes were analyzed by scanning electron microscopy and cell-adhesion, flexural-strength, and tear-strength assays. The results show that the cell-adhesion and mechanical properties of the composite membranes improved with increasing β-TCP and CS contents, yielding suitable levels of the adhesion of cells and adequate mechanical stability to ensure successful GBR. The CS adhered to the microsized β-TCP, which was distributed uniformly in the composite membranes. The β-TCP and CS have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility. This study demonstrates that β-TCP/CS/CG composite membranes are good candidates for GBR membranes in future applications.
在这项研究中，我们分析了壳聚糖（CS）和β-磷酸三钙[β-TCP，Ca（3）（PO（4））（2）]浓度变化对胶原（CG）基质用于引导骨再生（GBR）的机械性能和细胞黏附性能的影响。研究了三种不同的 CS 浓度（0.5%、1%和 2%）和五种不同含量的β-TCP（0%、17%、29%、38%和 44%）。通过扫描电子显微镜和细胞黏附、弯曲强度和撕裂强度测试分析复合膜。结果表明，随着β-TCP 和 CS 含量的增加，复合膜的细胞黏附性和机械性能得到改善，细胞黏附性达到适宜水平，机械稳定性也足够高，从而确保成功的 GBR。CS 黏附在微尺寸的β-TCP 上，β-TCP 在复合膜中均匀分布。β-TCP 和 CS 对细胞形态、活力和增殖没有负面影响，具有良好的生物相容性。本研究表明，β-TCP/CS/CG 复合膜是未来 GBR 膜的良好候选材料。
