Institute for Nuclear Chemistry, University of Mainz, Mainz, Germany.
Anal Bioanal Chem. 2012 Oct;404(6-7):1887-95. doi: 10.1007/s00216-012-6329-4. Epub 2012 Aug 24.
Boron determination in blood and tissue samples is a crucial task especially for treatment planning, preclinical research, and clinical application of boron neutron capture therapy (BNCT). Comparison of clinical findings remains difficult due to a variety of analytical methods, protocols, and standard reference materials in use. This paper addresses the comparability of inductively coupled plasma mass spectrometry, quantitative neutron capture radiography, and prompt gamma activation analysis for the determination of boron in biological samples. It was possible to demonstrate that three different methods relying on three different principles of sample preparation and boron detection can be validated against each other and yield consistent results for both blood and tissue samples. The samples were obtained during a clinical study for the application of BNCT for liver malignancies and therefore represent a realistic situation for boron analysis.
血液和组织样本中的硼测定是一项至关重要的任务，特别是对于硼中子俘获治疗（BNCT）的治疗计划、临床前研究和临床应用。由于临床应用中使用了各种分析方法、方案和标准参考物质，因此临床结果的比较仍然很困难。本文介绍了电感耦合等离子体质谱法、定量中子俘获放射照相术和瞬发伽马激活分析在生物样本中硼测定的可比性。结果表明，三种不同的方法，它们基于三种不同的样品制备和硼检测原理，可以相互验证，并对血液和组织样本产生一致的结果。这些样本是在 BNCT 应用于肝脏恶性肿瘤的临床研究中获得的，因此代表了硼分析的实际情况。
