Institute of Bioengineering and Swiss Institute of Experimental, Cancer Research (ISREC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland.
Angiogenesis. 2012 Dec;15(4):643-55. doi: 10.1007/s10456-012-9287-8. Epub 2012 Aug 24.
Therapeutic regulation of tissue vascularization has appeared as an attractive approach to treat a number of human diseases. In vivo neovascularization assays that reflect physiological and pathological formation of neovessels are important in this effort. In this report we present an assay where the effects of activators and inhibitors of angiogenesis can be quantitatively and qualitatively measured. A provisional matrix composed of collagen I and fibrin was formed in a plastic cylinder and implanted onto the chick chorioallantoic membrane. A nylon mesh separated the implanted matrix from the underlying tissue to distinguish new from pre-existing vessels. Vascularization of the matrix in response to fibroblast growth factor-2 or platelet-derived growth factor-BB was scored in a double-blinded manner, or vessel density was measured using a semi-automated image analysis procedure. Thalidomide, fumagillin, U0126 and TGFβ inhibited neovessel growth while hydrocortisone exerted a negative and wortmannin a toxic effect on the pre-existing vasculature. This quantitative, inexpensive and rapid in vivo angiogenesis assay might be a valuable tool in screening and characterizing factors that influence wound or tumor induced vascularization and in assessing their effects on the normal vasculature.
组织血管生成的治疗调节已成为治疗多种人类疾病的一种有吸引力的方法。在反映新血管生理和病理形成的体内新生血管生成测定中，这一方法非常重要。在本报告中，我们提出了一种可以定量和定性测量血管生成激活剂和抑制剂作用的测定方法。将胶原 I 和纤维蛋白组成的临时基质在塑料圆柱中形成，并植入鸡胚绒毛尿囊膜上。尼龙网将植入的基质与下面的组织隔开，以区分新形成的和预先存在的血管。以双盲方式对基质对成纤维细胞生长因子-2 或血小板衍生生长因子-BB 的血管生成反应进行评分，或使用半自动图像分析程序测量血管密度。沙利度胺、法呢基转移酶抑制剂、U0126 和 TGFβ 抑制新血管生成，而氢化可的松对预先存在的血管系统产生负向作用，而渥曼青霉素则产生毒性作用。这种定量的、廉价的、快速的体内血管生成测定方法可能是筛选和鉴定影响创伤或肿瘤诱导血管生成的因素以及评估它们对正常血管系统影响的有用工具。
