Kolesnichenko T S, Medvinskiĭ A L
Biull Eksp Biol Med. 1990 Aug;110(8):190-2.
The explants from 17-day old embryos of A and C57BL mice were used for long-term organ cultures. The following series of explants were studied: 1) the intact control explants; 2) the explants treated by NMU; 3) the explants treated by BP; 4) the explants isolated from mesenchyma (M); 5) the explants isolated from M and treated by NMU; 6) the explants isolated from M and treated by BP. The survival of explants treated by NMU or BP did not differ from the intact control explants (p less than 0.1). The removal of M decreased the survival only in the explants from the distal RT of A mice (p 0.01). The survival of explants isolated from M and treated by NMU or BP was significantly lower than it was in intact explants (p less than 0.01-0.001). Thus, the destruction of epithelial-mesenchymal interaction induced by removal of M can modulate the toxic effect of pulmonotropic carcinogens.
取自A系和C57BL系小鼠17日龄胚胎的外植体用于长期器官培养。研究了以下一系列外植体：1）完整对照外植体；2）经N-甲基-N-亚硝基脲（NMU）处理的外植体；3）经苯并芘（BP）处理的外植体；4）从间充质（M）分离的外植体；5）从M分离并经NMU处理的外植体；6）从M分离并经BP处理的外植体。经NMU或BP处理的外植体的存活率与完整对照外植体无差异（p小于0.1）。去除M仅降低了A系小鼠远端呼吸道（RT）外植体的存活率（p = 0.01）。从M分离并经NMU或BP处理的外植体的存活率显著低于完整外植体（p小于0.01至0.001）。因此，去除M诱导的上皮-间充质相互作用的破坏可调节亲肺致癌物的毒性作用。
