Kolesnichenko T S, Medvinskiĭ A L
Ontogenez. 1989 Jul-Aug;20(4):381-7.
Development of respiratory epithelium (RE) rudiment was studied in tissue culture after removal of mesenchyme (M) and respiratory tract (RT) in 13 days old embryos of A and C57BL mice. During long-term cultivation of intact RT, organotypic structures (branching bronchioles, alveolus-like cavities) developed. No epithelial organotypic structures developed in the presence of single M cells; explants were represented by layers of cubic epithelium. During long-term cultivation foci of atypical growth consisting of intensively proliferating basophilic cells with high nucleocytoplasmic ratio appeared in these explants. Regions of planocellular metaplasia with or without keratinization could be found in these foci. The frequency of atypical proliferates depended on the strain of donor mice and on the region of the explanted RT.
在去除A系和C57BL系小鼠13日龄胚胎的间充质（M）和呼吸道（RT）后，于组织培养中研究了呼吸上皮（RE）原基的发育情况。在完整呼吸道的长期培养过程中，出现了器官样结构（分支细支气管、肺泡样腔）。在仅有单个间充质细胞存在时，未形成上皮器官样结构；外植体由立方上皮层组成。在这些外植体的长期培养过程中，出现了由核质比高的嗜碱性细胞密集增殖构成的非典型生长灶。在这些病灶中可发现有或无角化的扁平细胞化生区域。非典型增殖的频率取决于供体小鼠的品系以及所取呼吸道外植体的部位。
