基于表面等离子体共振显微镜的单细胞-底物相互作用绘图。
Mapping single-cell-substrate interactions by surface plasmon resonance microscopy.
机构信息
Center for Bioelectronics and Biosensors, Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
出版信息
Langmuir. 2012 Sep 18;28(37):13373-9. doi: 10.1021/la301712h. Epub 2012 Sep 4.
Abstract
We report the imaging of the cell-substrate adhesion of a single cell with subcellular spatial resolution. Osmotic pressure was introduced to provide a controllable mechanical stimulation to the cell attached to a substrate, and high-resolution surface plasmon resonance microscopy was used to map the response of the cell, from which local cell-substrate adhesion was determined. In addition to high spatial resolution, the approach is noninvasive and fast and allows for the continuous mapping of cell-substrate interactions and single-cell movements.
摘要
我们报告了具有亚细胞空间分辨率的单个细胞与细胞基质黏附的成像。渗透压被引入到与基底相连的细胞，以提供可控的机械刺激，并用高分辨率的表面等离子体共振显微镜来绘制细胞的反应，从而确定局部细胞-基质的黏附。除了高空间分辨率外，该方法还具有非侵入性、快速的特点，并允许连续绘制细胞-基质相互作用和单细胞运动。
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