Center for Bioelectronics and Biosensors, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16028-32. doi: 10.1073/pnas.1005264107. Epub 2010 Aug 26.
We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect the binding of the viral particles to surfaces functionalized with and without antibodies. We show that the intensity of the particle image is related to the mass of the particle, from which we determine the mass and mass distribution of influenza viral particles with a mass detection limit of approximately 1 ag (or 0.2 fg/mm(2)). This work demonstrates a multiplexed method to measure the masses of individual viral particles and to study the binding activity of the viral particles.
我们通过高分辨率表面等离子体共振显微镜报告了在溶液中单病毒颗粒的无标记成像、检测和质量/尺寸测量。病毒颗粒沿金属表面传播的等离子体波的衍射产生了单个颗粒的图像，这使我们能够检测到病毒颗粒与带有和不带抗体的表面的结合。我们表明，颗粒图像的强度与颗粒的质量有关，由此我们确定了流感病毒颗粒的质量和质量分布，其质量检测限约为 1 ag（或 0.2 fg/mm(2)）。这项工作展示了一种用于测量单个病毒颗粒质量和研究病毒颗粒结合活性的多重方法。
