Zhou Xinyu, Porter Ryan, Zhou Xiaoyan, Jiang Jiapei, Braswell Brandyn, Wan Zijian, Zhang Pengfei, Wang Shaopeng
Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, AZ, 85287, USA.
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, 85287, USA.
Small. 2025 Aug;21(31):e2501394. doi: 10.1002/smll.202501394. Epub 2025 Jun 1.
Label-free plasmonic cell force microscopy is developed to reveal cell exerted force at diffraction-limited spatial resolution. By quantifying cell-substrate interaction dynamics in real-time through plasmonic scattering imaging, the spatial and temporal evolutions of cellular forces are accurately mapped. To demonstrate the capability of the technology, cardiomyocyte force evolution and loading rates are measured with millisecond resolution. Furthermore, cell force responses to nicotinic receptor activation are monitored and observed heterogenic cell force changes among a population of cells, underscoring the versatility and potential impact of this label-free approach.
无标记等离子体细胞力显微镜技术的开发旨在以衍射极限空间分辨率揭示细胞施加的力。通过等离子体散射成像实时量化细胞与基质的相互作用动态，精确绘制细胞力的时空演变。为了证明该技术的能力，以毫秒分辨率测量了心肌细胞的力演变和加载速率。此外，监测了细胞对烟碱受体激活的力反应，并观察到细胞群体中细胞力的异质性变化，突出了这种无标记方法的多功能性和潜在影响。
