School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Biosensors (Basel). 2024 Oct 30;14(11):524. doi: 10.3390/bios14110524.
Surface plasmon resonance (SPR) biosensors are extensively utilized for analyzing molecular interactions due to their high sensitivity and label-free detection capabilities. Recent innovations in surface-sensitive biosensors with dielectric surfaces address the inherent limitations associated with traditional gold surfaces, such as thermal effects and biocompatibility issues, which can impede broader applications. This review examines state-of-the-art biosensor configurations, including total internal reflection, optical waveguide, photonic crystal resonators, Bloch surface wave biosensors, and surface electrochemical biosensors, which can enhance analyte signals and augment the molecular detection efficiency at the sensor interface. These technological advancements not only improve the resolution of binding kinetics analysis and single-molecule detection but also extend the analytical capabilities of these systems. Additionally, this review explores prospective advancements in augmenting field enhancement and incorporating multimodal sensing functionalities, emphasizing the significant potential of these sophisticated biosensing technologies to profoundly enhance our understanding of molecular interactions.
表面等离子体共振（SPR）生物传感器由于其高灵敏度和无标记检测能力而被广泛用于分析分子相互作用。具有介电表面的表面敏感生物传感器的最新创新解决了与传统金表面相关的固有局限性，例如热效应和生物相容性问题，这些问题会阻碍更广泛的应用。本综述考察了最先进的生物传感器配置，包括全内反射、光波导、光子晶体谐振器、布洛赫表面波生物传感器和表面电化学生物传感器，这些配置可以增强分析物信号并提高传感器界面处的分子检测效率。这些技术进步不仅提高了结合动力学分析和单分子检测的分辨率，还扩展了这些系统的分析能力。此外，本综述探讨了增强场增强和结合多模态传感功能的预期进展，强调了这些复杂生物传感技术具有显著的潜力，可以深刻增强我们对分子相互作用的理解。
