Department of Pediatrics, Jilin University, Changchun, China.
Ann Allergy Asthma Immunol. 2012 Sep;109(3):201-7. doi: 10.1016/j.anai.2012.06.017. Epub 2012 Jul 17.
Allergic lung inflammation is mediated by allergen-specific T responses, which are negatively regulated by regulatory T cells (Tregs). Previous studies have reported that inoculation of indigenous Clostridium species in the early lives of mice can induce Tregs that colonize the colon. However, whether inoculation of C leptum alone in adult mice could induce systemic Treg responses and inhibit allergic airway inflammation remains unclear.
To investigate the effect of oral administration of C leptum on systemic Treg responses and allergic airway inflammation in a mouse model of asthma.
Adult BABL/c mice were injected with ovalbumin to induce asthma and treated orally with C leptum or vehicle daily for 2 weeks. The numbers of Foxp3(+)CD4(+)CD25(+) Tregs in both the spleen and mediastinal lymph nodes were examined by flow cytometry. After allergen challenge, the airway hyperresponsiveness of individual mice was measured, and the numbers of inflammatory infiltrates and the levels of cytokines in bronchoalveolar lavage fluids ere determined.
Oral feeding with C leptum increased the percentage and total number of Tregs in the spleens and mediastinal lymph nodes at 14 days after inoculation and attenuated allergen-induced airway hyperresponsiveness and inflammation by inhibiting inflammatory cytokine production but enhancing interleukin 10 and transforming growth factor β1 production in the lungs.
Oral treatment with C leptum can attenuate induced allergic airway inflammation in adult mice.
过敏性肺炎症由过敏原特异性 T 反应介导，而调节性 T 细胞（Tregs）可负向调节 T 反应。先前的研究报道，在小鼠的早期生活中接种本土梭菌可诱导定植于结肠的 Tregs。然而，在成年小鼠中单独接种 C. leptum 是否能诱导全身 Treg 反应并抑制过敏性气道炎症尚不清楚。
研究 C. leptum 口服给药对哮喘小鼠模型中全身 Treg 反应和过敏性气道炎症的影响。
成年 BABL/c 小鼠用卵清蛋白注射诱导哮喘，并每天口服 C. leptum 或载体治疗 2 周。通过流式细胞术检测脾和纵隔淋巴结中 Foxp3(+)CD4(+)CD25(+)Tregs 的数量。在过敏原攻击后，测量个体小鼠的气道高反应性，并测定支气管肺泡灌洗液中的炎症浸润细胞数量和细胞因子水平。
C. leptum 口服喂养在接种后 14 天增加了脾脏和纵隔淋巴结中 Tregs 的百分比和总数，并通过抑制炎症细胞因子的产生，增强白细胞介素 10 和转化生长因子 β1 的产生，减轻过敏原诱导的气道高反应性和炎症。
C. leptum 口服治疗可减轻成年小鼠诱导的过敏性气道炎症。
