Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
Domest Anim Endocrinol. 2013 Jan;44(1):19-25. doi: 10.1016/j.domaniend.2012.07.003. Epub 2012 Aug 10.
Equine insulin resistance is important because of its association with laminitis. The insulin-response test is described to diagnose insulin resistance in clinical settings. Practitioners may be reluctant to perform this test because of the time needed for the test and the fear of inducing hypoglycemia. The objective of the study was to compare a 2-step insulin-response test with a complete insulin-response test. A complete insulin-response test was performed on 6 insulin-resistant horses and 6 controls. A 2-step insulin-response test consisting of an intravenous injection of 0.1 IU/kg human insulin and blood glucose determination at 0 and 30 min after injection was performed on the same horses. Times to reach a 50% reduction of glucose baseline were compared between tests and horses. All the horses tolerated both tests well. No significant difference was observed between baseline glucose concentrations of insulin-resistant horses and controls (P = 0.09). Time to reach 50% reduction of glucose baseline for controls was not significantly different with the use of the complete insulin-response test or the 2-step test (P = 0.98). For insulin-resistant horses, the time to reach 50% reduction of glucose baseline with the use of the 2-step test was significantly longer than for controls (P = 0.004). With a cut-off time of 30 min, the 2-step test had the same characteristics as the complete test. The 2-step test provided a safe, rapid, and low-cost method to diagnose insulin resistance in horses in a clinical setting.
马的胰岛素抵抗很重要，因为它与蹄叶炎有关。胰岛素反应试验被描述为在临床环境中诊断胰岛素抵抗。由于该试验需要时间，并且担心会引起低血糖，从业者可能不愿意进行该试验。本研究的目的是比较两步胰岛素反应试验和完整的胰岛素反应试验。对 6 匹胰岛素抵抗马和 6 匹对照马进行了完整的胰岛素反应试验。对同一匹马进行了两步胰岛素反应试验，包括静脉注射 0.1IU/kg 人胰岛素和注射后 0 和 30 分钟时的血糖测定。比较了两种试验和马之间达到葡萄糖基线降低 50%的时间。所有的马都很好地耐受了这两种试验。胰岛素抵抗马和对照马的基础血糖浓度之间没有观察到显著差异（P = 0.09）。使用完整的胰岛素反应试验或两步试验时，对照马达到葡萄糖基线降低 50%的时间没有显著差异（P = 0.98）。对于胰岛素抵抗的马，使用两步试验达到葡萄糖基线降低 50%的时间明显长于对照马（P = 0.004）。使用 30 分钟的截止时间，两步试验与完整试验具有相同的特征。两步试验为在临床环境中诊断马的胰岛素抵抗提供了一种安全、快速且低成本的方法。
