Yang Jie, Li Jia-Xiang, Zhang Fei, Zhao Xin-Qing
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Eng Microbiol. 2022 Dec 16;3(2):100065. doi: 10.1016/j.engmic.2022.100065. eCollection 2023 Jun.
Rut-C-30 is a well-known robust producer of cellulolytic enzymes, which are used to degrade lignocellulosic biomass for the sustainable production of biofuels and biochemicals. However, studies of its secondary metabolism and regulation remain scarce. Ypr1 was previously described as a regulator of the biosynthesis of the yellow pigment sorbicillin (a bioactive agent with great pharmaceutical interest) in and several other fungi. However, the manner in which this regulator affects global gene transcription has not been explored. In this study, we report the effect of Ypr1 on the regulation of both the secondary and primary metabolism of Rut-C30. A global gene transcription profile was obtained using a comparative transcriptomic analysis of the wild-type strain Rut-C-30 and its deletion mutant. The results of this analysis suggest that, in addition to its role in regulating sorbicillin and the major extracellular (hemi)cellulases, Ypr1 also affects the transcription of genes encoding several other secondary metabolites. Although the primary metabolism of ∆ became less active compared with that of Rut-C-30, several gene clusters involved in its secondary metabolism were activated, such as the gene clusters for the biosynthesis of specific polyketides and non-ribosomal peptides, together with the "sorbicillinoid-cellulase" super cluster, indicating that specific secondary metabolites and cellulases may be co-regulated in Rut-C-30. The results presented in this study may benefit the development of genetic engineering strategies for the production of sorbicillin by Rut-C-30, and provide insights for enhancing sorbicillin production in other filamentous fungal producers.
Rut-C-30是一种著名的纤维素分解酶高产菌株，这些酶用于降解木质纤维素生物质，以可持续地生产生物燃料和生物化学品。然而，对其次级代谢和调控的研究仍然很少。Ypr1此前在[具体文献未提及]和其他几种真菌中被描述为黄色色素山梨素（一种具有重大药学意义的生物活性剂）生物合成的调节因子。然而，这种调节因子影响全局基因转录的方式尚未得到探索。在本研究中，我们报告了Ypr1对Rut-C30次级和初级代谢调控的影响。通过对野生型菌株Rut-C-30及其缺失突变体进行比较转录组分析，获得了全局基因转录谱。该分析结果表明，除了在调节山梨素和主要细胞外（半）纤维素酶方面的作用外，Ypr1还影响编码其他几种次级代谢产物的基因的转录。尽管与Rut-C-30相比，∆的初级代谢活性降低，但参与其次级代谢的几个基因簇被激活，例如特定聚酮化合物和非核糖体肽生物合成的基因簇，以及“山梨素样纤维素酶”超级簇，这表明特定的次级代谢产物和纤维素酶可能在Rut-C-30中共同调节。本研究结果可能有助于开发通过Rut-C-30生产山梨素的基因工程策略，并为提高其他丝状真菌生产者中山梨素的产量提供见解。
