School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1728-1733. doi: 10.1099/ijs.0.045906-0. Epub 2012 Aug 24.
Two actinomycete strains, BK125(T) and BK199(T), isolated from a hay meadow soil sample were investigated to determine their taxonomic position using a polyphasic approach. The isolates produced greenish-yellow and light green aerial mycelium on oatmeal agar, respectively. They contained anteiso-C15 : 0, iso-C15 : 0 and C16 : 0 as the major fatty acids, and MK-9 (H6) and MK-9 (H8) as the predominant isoprenoid quinones. Phylogenetic analysis of the 16S rRNA gene sequences showed that the isolates formed distinct phyletic lines towards the periphery of the Streptomyces prasinus subclade. Analysis of DNA-DNA relatedness between the two isolates showed that they belonged to different genomic species. The organisms were also distinguished from one another and from type strains of species classified in the S. prasinus subclade using a combination of genotypic and phenotypic properties. On the basis of these data, it is proposed that the isolates be assigned to the genus Streptomyces as Streptomyces chlorus sp. nov. and Streptomyces viridis sp. nov. with isolates BK125(T) ( = KACC 20902(T) = CGMCC 4.5798(T)) and BK199(T) ( = KACC 21003(T) = CGMCC 4.6824(T)) as the respective type strains.
两株放线菌菌株 BK125(T) 和 BK199(T) 从干草草坪土壤样本中分离出来，通过多相研究来确定它们的分类地位。这些分离株在燕麦琼脂上分别产生了绿黄色和浅绿色的气生菌丝体。它们含有anteiso-C15:0、iso-C15:0 和 C16:0 作为主要脂肪酸，MK-9 (H6) 和 MK-9 (H8) 作为主要异戊二烯醌。16S rRNA 基因序列的系统发育分析表明，这些分离株在链霉菌属的 S. prasinus 亚群的外围形成了独特的进化枝。对两个分离株之间的 DNA-DNA 同源性分析表明，它们属于不同的基因组种。这些生物还通过基因型和表型特性的组合与 S. prasinus 亚群中分类的种的类型菌株以及彼此之间区分开来。基于这些数据，建议将这些分离株分配到链霉菌属中，作为新种 Streptomyces chlorus sp. nov. 和 Streptomyces viridis sp. nov.，分离株 BK125(T)（ = KACC 20902(T) = CGMCC 4.5798(T)) 和 BK199(T)（ = KACC 21003(T) = CGMCC 4.6824(T)) 分别作为相应的模式株。
