School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2945-2950. doi: 10.1099/ijs.0.047050-0. Epub 2013 Jan 25.
A novel actinomycete, strain FX61(T), was isolated from a saline sample collected from the Inner Mongolian Autonomous Region in China and subjected to a taxonomic study using a polyphasic approach. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 1 H, C16 : 0, iso-C14 : 0 and anteiso-C17 : 0. The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides and an unidentified phospholipid. The G+C content of the genomic DNA was 72 mol%. The 16S rRNA gene sequence of the isolate had greater than 98 % similarity with those of Streptomyces griseoincarnatus ATCC 23623(T) (98.2 %), Streptomyces labedae DSM 41446(T) (98.2 %), Streptomyces variabilis ATCC 19815(T) (98.2 %), Streptomyces erythrogriseus ATCC 27427(T) (98.2 %), Streptomyces matensis ATCC 23935(T) (98.2 %), Streptomyces althioticus ATCC 19724(T) (98.2 %) and Streptomyces luteosporeus ATCC 33049(T) (98.0 %), showing that the novel strain should be assigned to the genus Streptomyces. DNA-DNA hybridizations with the seven above-mentioned members of the genus Streptomyces showed 29.8, 28.5, 27.0, 25.5, 25.0, 23.5 and 22.0 % relatedness, respectively. On the basis of the phenotypic characteristics and genotypic distinctiveness, strain FX61(T) should be classified as a novel species of the genus Streptomyces, for which the name Streptomyces wuyuanensis sp. nov. is proposed. The type strain is FX61(T) (= CGMCC 4.7042(T) = KCTC 29112(T)).
一株新的放线菌 FX61(T)，从中国内蒙古自治区的一个盐样本中分离出来，通过多相分类学研究进行了分类学研究。主要的类异戊二烯醌为 MK-9(H6)、MK-9(H8)和 MK-9(H4)。主要脂肪酸为 iso-C16:0、anteiso-C15:0、iso-C15:0、iso-C16:1 H、C16:0、iso-C14:0 和 anteiso-C17:0。磷脂谱含有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、两种磷脂酰肌醇甘露糖苷和一种未鉴定的磷脂。基因组 DNA 的 G+C 含量为 72mol%。该分离物的 16S rRNA 基因序列与灰色链霉菌 ATCC 23623(T)（98.2%）、拉贝链霉菌 DSM 41446(T)（98.2%）、变铅青链霉菌 ATCC 19815(T)（98.2%）、红褐链霉菌 ATCC 27427(T)（98.2%）、马氏链霉菌 ATCC 23935(T)（98.2%）、硫色链霉菌 ATCC 19724(T)（98.2%）和黄色链霉菌 ATCC 33049(T)（98.0%）的相似度大于 98%，表明该新菌株应属于链霉菌属。与上述七种链霉菌属的成员进行 DNA-DNA 杂交，其相关性分别为 29.8%、28.5%、27.0%、25.5%、25.0%、23.5%和 22.0%。基于表型特征和基因型特征，FX61(T)菌株应归类为链霉菌属的一个新种，命名为链霉菌武源亚种。模式菌株为 FX61(T)=CGMCC 4.7042(T)=KCTC 29112(T)。
