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实时单蛋白纳机械质谱分析。

Single-protein nanomechanical mass spectrometry in real time.

机构信息

Kavli Nanoscience Institute and Departments of Physics, Applied Physics, and Bioengineering, California Institute of Technology, MC 149-33, Pasadena, California 91125 USA.

出版信息

Nat Nanotechnol. 2012 Sep;7(9):602-8. doi: 10.1038/nnano.2012.119. Epub 2012 Aug 26.

DOI:10.1038/nnano.2012.119
PMID:22922541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3435450/
Abstract

Nanoelectromechanical systems (NEMS) resonators can detect mass with exceptional sensitivity. Previously, mass spectra from several hundred adsorption events were assembled in NEMS-based mass spectrometry using statistical analysis. Here, we report the first realization of single-molecule NEMS-based mass spectrometry in real time. As each molecule in the sample adsorbs on the resonator, its mass and position of adsorption are determined by continuously tracking two driven vibrational modes of the device. We demonstrate the potential of multimode NEMS-based mass spectrometry by analysing IgM antibody complexes in real time. NEMS-based mass spectrometry is a unique and promising new form of mass spectrometry: it can resolve neutral species, provide a resolving power that increases markedly for very large masses, and allow the acquisition of spectra, molecule-by-molecule, in real time.

摘要

纳米机电系统（NEMS）谐振器可以非常灵敏地检测质量。此前，已经使用统计分析方法在基于 NEMS 的质谱仪中组装了来自数百次吸附事件的质谱。在这里，我们报告了首次实时实现基于单分子的 NEMS 质谱。当样品中的每个分子吸附在谐振器上时，通过连续跟踪设备的两个驱动振动模式，可以确定其质量和吸附位置。我们通过实时分析 IgM 抗体复合物展示了基于多模式 NEMS 的质谱的潜力。基于 NEMS 的质谱是一种独特且有前途的新型质谱：它可以解析中性物质，提供对非常大质量明显增加的分辨率，并允许实时逐个分子地获取谱图。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/9ab5337cbe21/nihms386392f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/116e43a943b2/nihms386392f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/d7afadc3b134/nihms386392f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/65b51419583d/nihms386392f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/9cdf2f85b1f0/nihms386392f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/9ab5337cbe21/nihms386392f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/116e43a943b2/nihms386392f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/d7afadc3b134/nihms386392f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/65b51419583d/nihms386392f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/9cdf2f85b1f0/nihms386392f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f4f/3435450/9ab5337cbe21/nihms386392f5.jpg

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