Key Laboratory of Functional Dairy Science of Beijing and Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P. R. China.
J Microbiol. 2013 Apr;51(2):183-8. doi: 10.1007/s12275-013-2076-2. Epub 2013 Apr 27.
In this study, we utilized the nematode Caenorhabditis elegans to assess potential life-expanding effect of Lactobacillus salivarius strain FDB89 (FDB89) isolated from feces of centenarians in Bama County (Guangxi, China). This study showed that feeding FDB89 extended the mean life span in C. elegans by up to 11.9% compared to that of control nematodes. The reduced reproductive capacities, pharyngeal pumping rate, growth, and increased superoxide dismutase (SOD) activity and XTT reduction capacity were also observed in FDB89 feeding worms. To probe the anti-aging mechanism further, we incorporated a food gradient feeding assay and assayed the life span of eat-2 mutant. The results demonstrated that the maximal life span of C. elegans fed on FDB89 was achieved at the concentration of 1.0 mg bacterial cells/plate, which was 10-fold greater than that of C. elegans fed on E. coli OP50 (0.1 mg bacterial cells/plate). However, feeding FDB89 could not further extend the life span of eat-2 mutant. These results indicated that FDB89 modulated the longevity of C. elegans in a dietary restriction-dependent manner and expanded the understanding of anti-aging effect of probiotics.
在这项研究中，我们利用秀丽隐杆线虫来评估从中国广西巴马县百岁老人粪便中分离出的唾液乳杆菌 FDB89（FDB89）的潜在延长寿命效应。结果表明，与对照组线虫相比，FDB89 喂养可将秀丽隐杆线虫的平均寿命延长 11.9%。在 FDB89 喂养的线虫中还观察到生殖能力降低、咽泵动率、生长减缓以及超氧化物歧化酶（SOD）活性和 XTT 还原能力增加。为了进一步探究其抗衰老机制，我们采用食物浓度梯度喂养实验，并检测了 eat-2 突变体的寿命。结果表明，FDB89 喂养的秀丽隐杆线虫在 1.0mg 细菌细胞/平板浓度时达到最大寿命，比用大肠杆菌 OP50（0.1mg 细菌细胞/平板）喂养的秀丽隐杆线虫长 10 倍。然而，FDB89 喂养不能进一步延长 eat-2 突变体的寿命。这些结果表明，FDB89 以饮食限制依赖的方式调节秀丽隐杆线虫的寿命，并扩展了对益生菌抗衰老作用的理解。
