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利用秀丽隐杆线虫模型研究益生菌 LAB 对铜绿假单胞菌的体外和体内防御作用。

In vitro and in vivo defensive effect of probiotic LAB against Pseudomonas aeruginosa using Caenorhabditis elegans model.

机构信息

a Department of Food Science and Biotechnology, School of Bioconvergence Science and Technology , Kangwon National University , Chuncheon , Republic of Korea.

b Department of Medical Biomaterials Engineering and Institute of Bioscience and Biotechnology , Kangwon National University , Chuncheon , Republic of Korea.

出版信息

Virulence. 2018;9(1):1489-1507. doi: 10.1080/21505594.2018.1518088.

DOI:10.1080/21505594.2018.1518088
PMID:30257614
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6177248/
Abstract

This study aimed to investigate in vitro and in vivo the probiotic characteristics of lactic acid bacteria (LAB) isolated from Korean traditional fermented foods. Caenorhabditis elegans  (C. elegans) was used for analytical assays of fertility, chemotaxis, life-span, worm-killing and bacterial colonization in the intestinal lumen of the worm. All 35 strains of LAB reduced fertility and slowed development in the worms. The worm-killing assay showed that LAB significantly increased the lifespan (P < 0.05) and reduced the susceptibility to virulent PA14; however, the heat-killed LAB did not. The bacterial colonization assay revealed that LAB proliferated and protected the gut of the worm against infection by Pseudomonas aeruginosa PA14. In addition, specific LAB Pediococcus acidilactici(P. acidilactici DM-9), Pediococcus brevis (L. brevis SDL1411), and Pediococcus pentosaceus (P. pentosaceus SDL1409) strains showed acid resistance (66-91%), resistance to pepsin (64-67%) and viability in simulated intestinal fluid (67-73%) based on in vitro probiotic analyses. Taken together, these results suggest that C. elegans may be a tractable model for screening efficient probiotics.

摘要

本研究旨在从韩国传统发酵食品中分离出的乳酸菌（LAB）进行体外和体内的益生菌特性研究。秀丽隐杆线虫（C. elegans）被用于生育力、趋化性、寿命、杀线虫和肠道腔内细菌定植的分析测定。35 株 LAB 均能降低线虫的生育力并减缓其发育速度。杀线虫试验表明，LAB 显著延长了线虫的寿命（P<0.05），降低了对毒性 PA14 的敏感性；然而，热灭活 LAB 则没有。细菌定植试验表明，LAB 增殖并保护线虫肠道免受铜绿假单胞菌 PA14 的感染。此外，特定的 LAB 植物乳杆菌（P. acidilactici DM-9）、短乳杆菌（L. brevis SDL1411）和戊糖片球菌（P. pentosaceus SDL1409）菌株在体外益生菌分析中表现出耐酸性（66-91%）、耐胃蛋白酶性（64-67%）和在模拟肠液中的活力（67-73%）。综上所述，这些结果表明秀丽隐杆线虫可能是一种用于筛选有效益生菌的可行模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/a0151a659218/kvir-09-01-1518088-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/1702abccd2e2/kvir-09-01-1518088-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/a577b33879b4/kvir-09-01-1518088-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/b1038ba4e5db/kvir-09-01-1518088-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/ac4a2ce0ea0b/kvir-09-01-1518088-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/a0151a659218/kvir-09-01-1518088-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/1702abccd2e2/kvir-09-01-1518088-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/a577b33879b4/kvir-09-01-1518088-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/b1038ba4e5db/kvir-09-01-1518088-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/ac4a2ce0ea0b/kvir-09-01-1518088-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e440/6177248/a0151a659218/kvir-09-01-1518088-g005.jpg

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