爱泼斯坦-巴尔病毒DNA在转化的淋巴细胞中被扩增。
Epstein-Barr virus DNA is amplified in transformed lymphocytes.
作者信息
Sugden B, Phelps M, Domoradzki J
出版信息
J Virol. 1979 Sep;31(3):590-5. doi: 10.1128/JVI.31.3.590-595.1979.
Abstract
Leukocytes isolated from two adult donors who lacked detectable antibodies to antigens associated with Epstein-Barr virus were exposed to an average of 0.02 to 0.1 DNA-containing particles of Epstein-Barr virus per cell and immediately clones in agarose. Within about 30 generations all transformed cell clones contained between 5 and 800 copies of viral DNA per cell. Only 1 in 10(4) to less than 1 in 10(5) of the cells of each clone release virus, and the frequency of release did not correlate with the average number of copies of viral DNA in the cells of each clone. One clone that had an average of five copies of viral DNA per cell was recloned, and the average number of copies in four of six subclones increased 15-to 50-fold while the subclones were being propagated sufficiently to study them. These results indicate that Epstein-Barr virus DNA can undergo amplification relative to cell DNA at different times after it transforms cells.
摘要
从两名对与爱泼斯坦 - 巴尔病毒相关的抗原缺乏可检测抗体的成年供体中分离出的白细胞，平均每个细胞暴露于0.02至0.1个含爱泼斯坦 - 巴尔病毒的DNA颗粒，并立即在琼脂糖中克隆。在大约30代内，所有转化细胞克隆每个cell细胞含有5至800个病毒DNA拷贝。每个克隆中只有1/10⁴至不到1/10⁵的细胞释放病毒，且释放频率与每个克隆细胞中病毒DNA的平均拷贝数无关。对一个平均每个细胞有五个病毒DNA拷贝的克隆进行再克隆，在六个亚克隆中的四个亚克隆进行充分传代以便研究时，其平均拷贝数增加了15至50倍。这些结果表明，爱泼斯坦 - 巴尔病毒DNA在转化细胞后的不同时间相对于细胞DNA可进行扩增。
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