Departments of Pathology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Korea.
Pathol Int. 2012 Sep;62(9):583-91. doi: 10.1111/j.1440-1827.2012.02845.x.
MicroRNA (miRNA) has a critical effect on tumorigenesis through post-transcriptional modification and is considered to be potential biomarkers for cancer diagnosis and treatment monitoring. We evaluated the expression pattern of three selected miRNAs (miR-21, miR-155, and let-7a) to evaluate their potential roles by quantitative reverse transcription-polymerase chain reaction using formalin-fixed and paraffin-embedded tissues of 63 surgically resected pulmonary neuroendocrine (NE) tumors (19 typical carcinoids (TCs), 6 atypical carcinoids (ACs), 19 large cell NE carcinomas (LCNECs), and 19 small cell lung carcinomas (SCLCs). Control amplification for U6 small nuclear RNA (U6) was performed in all samples. Normalized Ct values were calculated (Ct(Experimental miRNA) -Ct(U6) ) for each case and recorded. The expression levels of miR-21 and miR-155 were significantly higher in high-grade NE carcinomas (LCNECs and SCLCs) than in carcinoid tumors (TCs and ACs) (each P < 0.001). The expression level of miR-21 in carcinoid tumors with lymph node metastasis was significantly higher than in carcinoid tumors without lymph node metastasis (P= 0.010). To the best of our knowledge, the present study is the first to examine the expression patterns of miR-21 and miR-155 as an adjunctive diagnostic tool or clinically relevant biomarkers for pulmonary NE tumors.
微小 RNA（miRNA）通过转录后修饰对肿瘤发生具有重要影响，被认为是癌症诊断和治疗监测的潜在生物标志物。我们通过定量逆转录聚合酶链反应评估了三种选定 miRNA（miR-21、miR-155 和 let-7a）的表达模式，使用福尔马林固定和石蜡包埋的 63 例手术切除的肺神经内分泌（NE）肿瘤组织（19 例典型类癌（TCs）、6 例非典型类癌（ACs）、19 例大细胞 NE 癌（LCNECs）和 19 例小细胞肺癌（SCLCs））来评估它们的潜在作用。所有样本均进行 U6 小核 RNA（U6）的对照扩增。计算每个病例的归一化 Ct 值（Ct（实验 miRNA）-Ct（U6））并记录。miR-21 和 miR-155 的表达水平在高级别 NE 癌（LCNECs 和 SCLCs）中明显高于类癌肿瘤（TCs 和 ACs）（均 P<0.001）。有淋巴结转移的类癌肿瘤中 miR-21 的表达水平明显高于无淋巴结转移的类癌肿瘤（P=0.010）。据我们所知，本研究首次检查了 miR-21 和 miR-155 的表达模式，作为肺 NE 肿瘤的辅助诊断工具或临床相关生物标志物。
